Here, we used a fluorophore-bound, porphyrin-targeted DNA aptamer to increase the sensitivity of capillary electrophoresis for the detection of a model porphyrin. The complex of the DNA aptamer with the porphyrin induced the transfer of energy from the fluorophore to the porphyrin, resulting in the quenching of fluorescence. Monitoring of the quenching of fluorescence by capillary electrophoresis allowed the model porphyrin to be detected at sub-nanomolar concentrations, which is a marked improvement over conventional direct-detection capillary electrophoresis approaches.

中文翻译：

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DNA适体和卟啉配合物的荧光猝灭，用于毛细管电泳的灵敏检测。

在这里，我们使用了荧光团绑定，卟啉靶向的DNA适体，以增加毛细管电泳检测模型卟啉的灵敏度。DNA适体与卟啉的复合物导致能量从荧光团转移到卟啉，从而导致荧光猝灭。通过毛细管电泳监测荧光猝灭，可以在亚纳摩尔浓度下检测模型卟啉，这是对常规直接检测毛细管电泳方法的显着改进。