The interaction between dietary valine (Val) and isoleucine (Ile) on growth, blood parameters, and tolerance against low salinity stress of Japanese flounder Paralichthys olivaceus was studied in a 60‐day feeding trial. Fish (initial body weight: 3.57 ± 0.05 g) were fed six experimental diets containing different proportions of Ile and Val, two levels of Ile 0% (LI) and 2% diet (HI), combine with three levels of Val 0% (LV), 0.83% (MV), and 2.27% diet (HV), respectively. The results revealed that significant interactive effect of Ile and Val was found on body weight gain and feed conversion ratio, also stimulative effect was found in high Ile groups. Meanwhile, interactive effect of these two amino acids was also observed on high‐density lipoprotein (HDL), the maximum of Ile with maximum of Val group has the highest concentration of HDL, which was significantly higher than other groups (p < .05). Moreover, no interaction was found on whole body proximate composition, muscle Ile and Val concentration, and tolerance ability against low salinity stress (reflected by LT₅₀). But high level of Ile combined with high level of Val diet altered tolerance ability against low salinity stress of Japanese flounder. It would be indicated that dietary Ile and Val interacted in the diet of Japanese flounder, and the flounders fed the diet supplemented with 2% Ile and 2.27% Val was superior to the other groups on growth and blood parameters, and tolerance against low salinity stress.

中文翻译：

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饮食缬氨酸和异亮氨酸之间的相互作用对日本比目鱼（Paraliichthys olivaceus）生长性能，血液参数和抗低盐胁迫的抗性

日粮比目鱼（Valparachthys olivaceus）的饮食缬氨酸（Val）和异亮氨酸（Ile）之间的相互作用对生长，血液参数和对低盐分胁迫的耐受性在60天的喂养试验中进行了研究。向鱼类（初始体重：3.57±0.05克）喂食六种实验饮食，其中分别包含不同比例的Ile和Val，两种水平的Ile 0％（LI）和2％饮食（HI），以及三种水平的Val 0％（ LV），0.83％（MV）和2.27％饮食（HV）。结果表明，Ile和Val对体重增加和饲料转化率具有显着的交互作用，高Ile组也具有刺激作用。同时，还观察到这两个氨基酸对高密度脂蛋白（HDL）的相互作用，Ile和Val组最高，HDL浓度最高，明显高于其他组（p <.05）。而且，在全身附近的成分，肌肉Ile和Val浓度以及对低盐度胁迫的耐受能力（LT ₅₀反映）上均未发现相互作用。但是高水平的Ile和高水平的Val饮食改变了对日本比目鱼低盐度胁迫的耐受能力。这表明日粮比目鱼的饮食中Ile和Val相互影响，饲喂日粮中添加2％Ile和2.27％Val的比目鱼在生长和血液参数以及对低盐度胁迫的耐受性方面均优于其他组。 。