Many chemotherapeutic regimens have been investigated for advanced unresectable and metastatic pancreatic cancer (PC), but with only minimal improvement in survival and prognosis. Here, we investigated anti-cancer function of free and nano-encapsulated hydroxytyrosol (Hyd) and curcumin (Cur), and its combinations (Hyd-Cur) on PANC-1 cell line. The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hyd and Cur were synthesized, and MTT assay was performed to evaluate cytotoxic effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur. Effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur were evaluated on viability, migration, morphological alterations, colony formation, and apoptosis on PANC-1 cells. We observed that free and nano-encapsulated Hyd, Cur, and Hyd-Cur significantly increased apoptosis rates as well as significantly decreased viability, migration, and colony formation in PANC-1 cells. According to our results, Hyd-Cur combination and nano-encapsulation therapy exerts more profound apoptotic and anti-proliferative effects on PANC-1 cells than free Hyd or Hyd monotherapy.

中文翻译：

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通过PLGA-PAA纳米包封增强羟基酪醇和姜黄素对PANC-1胰腺癌细胞系的抗癌效力

许多化疗方案已被研究用于晚期不可切除和转移性胰腺癌 (PC)，但对生存和预后的改善很小。在这里，我们研究了游离和纳米包裹的羟基酪醇 (Hyd) 和姜黄素 (Cur) 及其组合 (Hyd-Cur) 在 PANC-1 细胞系上的抗癌功能。合成了聚丙交酯-共-乙交酯-共聚丙烯酸（PLGA-co-PAA）纳米包封的Hyd和Cur，并通过MTT法评估游离和纳米包封的Hyd、Cur和Hyd-库尔。评估游离和纳米封装的 Hyd、Cur 和 Hyd-Cur 对 PANC-1 细胞的活力、迁移、形态学改变、集落形成和细胞凋亡的影响。我们观察到游离和纳米封装的 Hyd、Cur、和 Hyd-Cur 显着增加了 PANC-1 细胞的凋亡率，并显着降低了活力、迁移和集落形成。根据我们的结果，与游离的 Hyd 或 Hyd 单一疗法相比，Hyd-Cur 组合和纳米封装疗法对 PANC-1 细胞产生更深远的凋亡和抗增殖作用。