The dietary composition of fish constitutes numerous immune and growth-promoting substance; during the current study, methionine was amended in the diet of Labeo rohita (L. rohita), in order to appraise the diverse modulatory effects of methionine supplementation. Fish were grouped into three on the basis of methionine as methionine supplemented group 1 (MSG1), methionine supplemented group 2 (MSG2), and CTRL (control group). We assessed the effects of methionine at the end. Captivating results were obtained from the methionine supplementing groups. The results depict significantly high (p < 0.05) innate immunity parameters, including myeloperoxidase, IgG concentrations, lysozyme, respiratory burst, and total bacteriolytic activity of complement in MSG2. Furthermore, ameliorate growth performances were also noticed such as high weight gain, FCE%, and body length in MSG2, respectively. We also measured high level of antioxidant digestive enzymes, gut length, and survival rate in MSG2 compared to the rest of the groups. The results were further validated by measuring the stress parameters, such as aspartate aminotransferase (AST), glucose, and hematocrit (HCT%), and the concentration remains low in MSG2. Lastly, after 90 days, fish were challenged with Aeromonas hydrophila, the highest survival was observed in the MSG2 and MSG1 compared to CTRL. These results ultimately give the baseline to the credible effects of methionine on L. rohita.

鱼的饮食组成构成了许多免疫和促进生长的物质。在当前的研究中，为了评估蛋氨酸补充剂的多种调节作用，对Labeo rohita（L. rohita）的饮食中的蛋氨酸进行了修改。根据蛋氨酸将鱼分为三个部分，分别为蛋氨酸补充组1（MSG1），蛋氨酸补充组2（MSG2）和CTRL（对照组）。最后，我们评估了蛋氨酸的作用。从蛋氨酸补充剂组获得了诱人的结果。结果显示明显较高（p<0.05）先天免疫参数，包括髓过氧化物酶，IgG浓度，溶菌酶，呼吸爆发和MSG2中补体的总溶菌活性。此外，还注意到改善的生长性能，例如分别在MSG2中高体重增加，FCE％和体长。与其他组相比，我们还测量了MSG2中高水平的抗氧化剂消化酶，肠道长度和存活率。通过测量压力参数，例如天冬氨酸转氨酶（AST），葡萄糖和血细胞比容（HCT％），进一步验证了结果，并且MSG2中的浓度仍然较低。最后，在90天后，鱼类受到嗜水气单胞菌的攻击，与CTRL相比，MSG2和MSG1的存活率最高。这些结果最终为蛋氨酸对罗伊塔氏菌的可信作用提供了基线。