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Phenylboronic Acid-conjugated Exosomes for Enhanced Anticancer Therapeutic Effect by Increasing Doxorubicin Loading Efficiency
Biotechnology and Bioprocess Engineering ( IF 2.5 ) Pub Date : 2021-01-26 , DOI: 10.1007/s12257-020-0107-5
Haneul Kim , Su Jin Kang , Won Jong Rhee

Anticancer drugs, including doxorubicin (DOX), have been widely used for cancer treatment, but these chemotherapeutic drugs have some issues to address because of their associated side effects, such as cytotoxicity. Various synthetic delivery vehicles for anticancer drugs have been developed to encapsulate and transfer them to cancer cells. However, these delivery vehicles also have high cytotoxicity and immunogenicity. Recently, exosomes have been highlighted as candidates for anticancer drug delivery vehicles with fewer side effects. Exosomes are nanosized particles that are produced from cells, and high concentrations of exosomes are already present in the human body. To develop exosome-based anticancer therapeutics, high drug concentrations should be loaded into exosomes. In this context, phenylboronic acid (PBA) was introduced and chemically conjugated to the exosome surface to load DOX to exosomes. Consequently, a high amount of DOX was efficiently loaded onto the PBA-conjugated exosomes. The DOX-loaded PBA-conjugated exosomes were applied to MDA-MB-231 breast cancer cells and showed enhanced cancer cell cytotoxicity compared to free DOX and DOX-loaded non-conjugated exosomes. Therefore, using PBA-conjugated exosomes for delivering DOX to cancer cells can be a promising strategy for effectively killing cancer cells because a high amount of DOX can be loaded and delivered.



中文翻译:

苯硼酸偶联的外泌体通过增加阿霉素的负载效率来增强抗癌治疗效果

包括阿霉素(DOX)在内的抗癌药物已被广泛用于癌症治疗,但是这些化学治疗药物由于其相关的副作用(例如细胞毒性)而需要解决一些问题。已经开发了用于抗癌药物的各种合成递送载体以将其封装并转移至癌细胞。但是,这些递送载体也具有高细胞毒性和免疫原性。近来,外泌体已被认为是具有较少副作用的抗癌药物递送载体的候选者。外泌体是由细胞产生的纳米级颗粒,人体中已经存在高浓度的外泌体。要开发基于外泌体的抗癌疗法,应将高浓度的药物加载到外泌体中。在这种情况下,引入苯硼酸(PBA)并将其化学偶联至外泌体表面,以将DOX负载至外泌体。结果,大量的DOX被有效地装载到缀合了PBA的外泌体上。将DOX负载的PBA偶联外泌体应用于MDA-MB-231乳腺癌细胞,与游离DOX和DOX负载的非偶联外泌体相比,癌细胞的细胞毒性增强。因此,使用PBA偶联的外来体将DOX递送至癌细胞可能是有效杀死癌细胞的有前途的策略,因为可以装载和递送大量的DOX。将DOX负载的PBA偶联外泌体应用于MDA-MB-231乳腺癌细胞,与游离DOX和DOX负载的非偶联外泌体相比,癌细胞的细胞毒性增强。因此,使用PBA偶联的外来体将DOX递送至癌细胞可能是有效杀死癌细胞的有前途的策略,因为可以装载和递送大量的DOX。将DOX负载的PBA偶联外泌体应用于MDA-MB-231乳腺癌细胞,与游离DOX和DOX负载的非偶联外泌体相比,癌细胞的细胞毒性增强。因此,使用PBA偶联的外来体将DOX递送至癌细胞可能是有效杀死癌细胞的有前途的策略,因为可以装载和递送大量的DOX。

更新日期:2021-01-28
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