Nanomaterials and nanotechnology have been widely used in various fields, such as materials, chemical engineering, optics, and other disciplines. In this paper, gold nanoparticles are prepared by the hydrothermal method and treated with polypyrrole coating (PPy-GNPs). Then, the anti-HER2 protein was conjugated to polypyrrole coated gold nanoparticles, and the nanomaterials are named anti-HER2-PPy@GNPs (anti-PPy@GNPs). Their diameter is about 80–90 nm, and they show a strong absorption peak in the near-infrared band, especially under 808 nm laser irradiation. In the MTT test experiment, anti-HER2-PPy@GNPs show a significant inhibitory effect on the SKOV-3 cell. Fluorescent cytoskeletal analysis of SKOV-3 cells and atomic force microscopy (AFM) of the mechanical characteristics of the cell surface revealed that the cell micro-structure, adhesion, and Young's modulus of anti-HER2-PPy@GNPs have changed under the near-infrared illumination. These results indicate anti-HER2-PPy@GNPs has an inhibitory effect on SKOV-3 cells. AFM detects the mechanical characteristics of SKOV-3 cells. Compared with the control group, Young's modulus is increased 52.7%, while the adhesion force is decreased 14.47%.

纳米材料和纳米技术已广泛用于各种领域，例如材料，化学工程，光学和其他学科。本文采用水热法制备了金纳米颗粒，并用聚吡咯涂层（PPy-GNPs）处理。然后，将抗-HER2蛋白缀合到聚吡咯涂层的金纳米颗粒上，并将纳米材料命名为抗-HER2-PPy @ GNPs（anti-PPy @ GNPs）。它们的直径约为80–90 nm，并且在近红外波段显示出很强的吸收峰，尤其是在808 nm激光照射下。在MTT测试实验中，抗HER2-PPy @ GNPs对SKOV-3细胞显示出明显的抑制作用。SKOV-3细胞的荧光细胞骨架分析和原子力显微镜（AFM）对细胞表面的机械特性的揭示表明，细胞的微观结构，粘附力，抗HER2-PPy @ GNPs的杨氏模量在近红外光照射下发生了变化。这些结果表明抗HER2-PPy @ GNP对SKOV-3细胞具有抑制作用。AFM检测SKOV-3电池的机械特性。与对照组相比，杨氏模量增加了52.7％，而粘附力降低了14.47％。