Antibiotic-resistance and bacterial bioburden are the threats to wound healing. Nanoparticles are a revolutionary advancement in nanomedicine owing to their antibacterial properties, to be used as a promising alternative to antibiotics. A wound dressing with antibacterial and anti-biofilm effects may be a plausible strategy in curbing wound exacerbation caused by bacterial infection. Silver nanoparticles (AgNPs) are known for their excellent antibacterial activity. In present study, biosynthesized AgNPs using spent mushroom substrate (SMS) as reducing agent while chitosan as stabilizing agent were successfully produced. The nanoparticles exhibited considerable antibacterial and anti-biofilm activities with higher sensitivity towards Gram-negative bacteria. These AgNPs were later incorporated into genipin-crosslinked gelatine hydrogels as a wound dressing. The hydrogels were characterized for their physical, rheological and swelling properties, besides in vitro release of AgNPs. The formulated hydrogels demonstrated an adequate release of the AgNPs with acceptable physical, rheological and swelling properties, suitable as a vehicle for wound drug delivery. The antibacterial and anti-biofilm of the hydrogels against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli were effective in inhibiting the growth of the selected bacteria with minimum inhibitory concentration (MIC) of 63 μg/mL. In summary, genipin-crosslinked gelatine hydrogels loaded with the AgNPs are a promising anti-microbial wound dressing, a potential strategy for combating biofilm of wound infections and accelerating healing.

抗生素耐药性和细菌负担是伤口愈合的威胁。由于其抗菌特性，纳米颗粒是纳米医学的革命性进步，被用作抗生素的有前途的替代品。具有抗菌和抗生物膜作用的伤口敷料可能是遏制细菌感染引起的伤口恶化的可行策略。银纳米颗粒（AgNPs）以其出色的抗菌活性而闻名。在本研究中，成功​​生产了用废蘑菇底物（SMS）作为还原剂而壳聚糖作为稳定剂的生物合成AgNPs。纳米颗粒表现出相当大的抗菌和抗生物膜活性，对革兰氏阴性细菌具有更高的敏感性。这些AgNPs随后作为伤口敷料掺入Genipin交联的明胶水凝胶中。除了体外释放AgNPs外，还对水凝胶的物理，流变和溶胀特性进行了表征。配制的水凝胶显示出具有可接受的物理，流变和溶胀特性的AgNP的充分释放，适合作为伤口药物输送的载体。水凝胶对细菌的抗菌和抗生物膜金黄色葡萄球菌，枯草芽孢杆菌，铜绿假单胞菌和大肠杆菌可有效抑制所选细菌的生长，最低抑制浓度（MIC）为63μg/ mL。总之，载有AgNPs的Genipin交联明胶水凝胶是一种有前途的抗微生物伤口敷料，是对抗伤口感染的生物膜并加速愈合的潜在策略。