Antibacterial Prenylated p-Hydroxybenzoic Acid Derivatives from Oberonia myosurus and Identification of Putative Prenyltransferases,Journal of Natural Products

当前位置： X-MOL 学术 › J. Nat. Prod. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Antibacterial Prenylated p-Hydroxybenzoic Acid Derivatives from Oberonia myosurus and Identification of Putative Prenyltransferases
Journal of Natural Products ( IF 3.3 ) Pub Date : 2021-01-25 , DOI: 10.1021/acs.jnatprod.0c01101
Fu-Cai Ren _{1,

2} , Li Liu _{1,

2} , Yong-Feng Lv _{1,

2} , Xue Bai ₁ , Qian-Jin Kang ₃ , Xiao-Jing Hu ₃ , Hong-Dan Zhuang ₁ , Liu Yang ₁ , Jiang-Miao Hu ₁ , Jun Zhou ₁

Affiliation

Twelve hitherto unknown tandem prenylated p-hydroxybenzoic acid derivatives, namely, oberoniamyosurusins A–L, together with five known derivatives, were isolated from an EtOH extract of the whole parts of the plant Oberonia myosurus. Compounds 10, 13, and 17 exhibited moderate inhibitory activity against Staphylococcus aureus subsp. aureus ATCC29213 with MIC₅₀ values ranging from 7.6 to 23 μg/mL. To determine the biosynthetic pathway of this class of tandem prenyl-substituted compounds, the full-length transcriptome of O. myosurus was sequenced, yielding 19.09 Gb of clean data and 10 949 nonredundant sequences. Two isoforms of p-hydroxybenzoic acid prenyltransferases were annotated and functionally characterized as the enzymes that might be involved in the biosynthesis of nervogenic acid (13) in Pichia pastoris.

中文翻译：

来自 Oberonia myosurus 的抗菌异戊二烯化对羟基苯甲酸衍生物和假定的异戊二烯转移酶的鉴定

从植物Oberonia myosurus的整个部分的乙醇提取物中分离出12 种迄今为止未知的串联异戊二烯化对羟基苯甲酸衍生物，即 oberoniamyosurusins A–L，以及五种已知的衍生物。化合物10，13和17表现出对中等抑制活性金黄色葡萄球菌亚种。aureus ATCC29213 的 MIC ₅₀值范围为 7.6 至 23 μg/mL。为了确定此类串联异戊二烯取代化合物的生物合成途径，O. myosurus的全长转录组测序，产生 19.09 Gb 的干净数据和 10 949 个非冗余序列。对羟基苯甲酸异戊二烯基转移酶的两种同工型进行了注释，并在功能上表征为可能参与毕赤酵母中神经原酸 ( 13 )生物合成的酶。

更新日期：2021-02-26

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11