Cartilage lesion is a common tissue defect and is challenging in clinical practice. Trauma-induced cellular senescence could decrease the chondrocyte capability of maintaining cartilage tissue regeneration. A previous investigation showed that, by controlling the cellular senescence, the cartilage regeneration can be significantly accelerated. Based on this finding, we design a novel hydrogel, Alg/MH-Sr, that combines metformin, an established drug for inhibiting senescence, and strontium, an effective anti-inflammatory material for cartilage tissue engineering. A RT-PCR test suggests the significant inhibitory effect of the hydrogel on senescent, apoptotic, oxidative, and inflammatory genes’ expression. Histological examinations demonstrate that the Alg/MH-Sr hydrogel accelerated cartilage repairment, and chondrocyte senescence was significantly inhibited. Our study demonstrates that the Alg/MH-Sr hydrogel is effective for cartilage defect treatment and provides a new clue in accelerating tissue repairment by inhibiting the senescence of cells and tissues.

中文翻译：

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海藻酸钠锶水凝胶包裹盐酸二甲双胍通过缓解细胞衰老再生软骨。

软骨损伤是常见的组织缺陷，并且在临床实践中具有挑战性。创伤引起的细胞衰老可能降低软骨细胞维持软骨组织再生的能力。先前的研究表明，通过控制细胞衰老，可以显着促进软骨再生。基于这一发现，我们设计了一种新型水凝胶Alg / MH-Sr，它结合了二甲双胍（一种已成熟的抑制衰老的药物）和锶（一种有效的抗炎材料，用于软骨组织工程）。RT-PCR测试表明水凝胶对衰老，凋亡，氧化和炎性基因表达具有明显的抑制作用。组织学检查表明，Alg / MH-Sr水凝胶可加速软骨修复，并且软骨细胞的衰老被明显抑制。我们的研究表明，Alg / MH-Sr水凝胶可有效治疗软骨缺损，并通过抑制细胞和组织的衰老为加速组织修复提供新的线索。