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PLLA Porous Microsphere-Reinforced Silk-Based Scaffolds for Auricular Cartilage Regeneration
ACS Omega ( IF 3.7 ) Pub Date : 2021-01-24 , DOI: 10.1021/acsomega.0c05890 Yan Zeng 1 , Xiaokai Li 2, 3 , Xia Liu 1 , Yuzhou Yang 2, 3 , Zhimin Zhou 2, 3 , Jincai Fan 1 , Haiyue Jiang 1
ACS Omega ( IF 3.7 ) Pub Date : 2021-01-24 , DOI: 10.1021/acsomega.0c05890 Yan Zeng 1 , Xiaokai Li 2, 3 , Xia Liu 1 , Yuzhou Yang 2, 3 , Zhimin Zhou 2, 3 , Jincai Fan 1 , Haiyue Jiang 1
Affiliation
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Microtia, frequently encountered in plastic surgery practice, is usually corrected by auricular reconstruction with prostheses or autologous cartilages. In recent decades, however, cartilage tissue engineering has been emerging as a promising alternative for its minimal invasion and low immunogenicity. As a critical factor for tissue engineering, scaffolds are expected to be sufficiently porous and stiff to facilitate chondrogenesis. In this work, we introduce novel poly-l-lactic acid (PLLA) porous microsphere-reinforced silk-based hybrid (SBH) scaffolds with a multihierarchical porous structure. The scaffolds are fabricated by embedding PLLA porous microspheres (PMs) into a blending matrix of silk fibroin (SF) and gelatin solution, followed by mixing with a degummed silk fiber mesh and freeze-drying process. Through adjusting the amount of PLLA PMs, the mechanical strength approximates to natural cartilage and also balanced physical properties were realized. Biological evaluations of SBH scaffolds, both in vitro and in vivo, were conducted and PM-free plain silk-based (PSB) scaffolds were applied as control. Overall, it suggests that the incorporation of PLLA PMs remarkably improves mechanical properties and the capability to promote chondrogenesis of SBH scaffolds, and that SBH scaffolds appear to be a promising construct for potential applications in auricular cartilage tissue engineering and relevant fields.
中文翻译：
PLLA多孔微球增强丝基支架用于耳软骨再生。
整形外科实践中经常遇到的小口畸形，通常可以通过假体或自体软骨的耳廓重建来纠正。然而，近几十年来，由于其最小的侵袭和低的免疫原性，软骨组织工程已经成为有希望的替代方案。作为组织工程的关键因素，支架应具有足够的多孔性和坚硬性，以促进软骨形成。在这项工作中，我们引入新的聚升-乳酸（PLLA）多孔微球增强的丝基杂化（SBH）支架，具有多层次的多孔结构。通过将PLLA多孔微球（PMs）嵌入到丝素蛋白（SF）和明胶溶液的混合基质中，然后与脱胶的丝纤维筛网混合并进行冷冻干燥，来制造支架。通过调节PLLA PMs的数量，机械强度接近天然软骨，并实现了平衡的物理性能。SBH支架的生物学评估，无论是在体内还是在体外，均采用不含PM的普通丝基（PSB）支架作为对照。总体而言，这表明加入PLLA PMs可以显着改善机械性能以及促进SBH支架软骨形成的能力，
更新日期：2021-02-02
中文翻译：
PLLA多孔微球增强丝基支架用于耳软骨再生。
整形外科实践中经常遇到的小口畸形，通常可以通过假体或自体软骨的耳廓重建来纠正。然而，近几十年来，由于其最小的侵袭和低的免疫原性，软骨组织工程已经成为有希望的替代方案。作为组织工程的关键因素，支架应具有足够的多孔性和坚硬性，以促进软骨形成。在这项工作中，我们引入新的聚升-乳酸（PLLA）多孔微球增强的丝基杂化（SBH）支架，具有多层次的多孔结构。通过将PLLA多孔微球（PMs）嵌入到丝素蛋白（SF）和明胶溶液的混合基质中，然后与脱胶的丝纤维筛网混合并进行冷冻干燥，来制造支架。通过调节PLLA PMs的数量，机械强度接近天然软骨，并实现了平衡的物理性能。SBH支架的生物学评估，无论是在体内还是在体外，均采用不含PM的普通丝基（PSB）支架作为对照。总体而言，这表明加入PLLA PMs可以显着改善机械性能以及促进SBH支架软骨形成的能力，
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