Canine cutaneous epitheliotropic T-cell lymphoma (CETL) and immune-mediated T-cell predominant dermatoses (IMD) share several clinical and histopathological features, but differ substantially in prognosis. The discrimination of ambiguous cases may be challenging, as diagnostic tests are limited and may prove equivocal. This study aimed to investigate transcriptional differences between CETL and IMD, as a basis for further research on discriminating diagnostic biomarkers. We performed 100bp single-end sequencing on RNA extracted from formalin-fixed and paraffin-embedded skin biopsies from dogs with CETL and IMD, respectively. DESeq2 was used for principal component analysis (PCA) and differential gene expression analysis. Genes with significantly different expression were analyzed for enriched pathways using two different tools. The expression of selected genes and their proteins was validated by RT-qPCR and immunohistochemistry. PCA demonstrated the distinct gene expression profiles of CETL and IMD. In total, 503 genes were upregulated, while 4986 were downregulated in CETL compared to IMD. RT-qPCR confirmed the sequencing results for 5/6 selected genes tested, while the protein expression detected by immunohistochemistry was not entirely consistent. Our study revealed transcriptional differences between canine CETL and IMD, with similarities to human cutaneous lymphoma. Differentially expressed genes are potential discriminatory markers, but require further validation on larger sample collections.

中文翻译：

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犬皮肤亲上皮性淋巴瘤和免疫介导的皮肤病之间的转录差异


犬皮肤亲上皮性 T 细胞淋巴瘤 (CETL) 和免疫介导的 T 细胞为主的皮肤病 (IMD) 具有一些共同的临床和组织病理学特征，但预后存在很大差异。对模棱两可的病例进行区分可能具有挑战性，因为诊断测试有限且可能模棱两可。本研究旨在调查 CETL 和 IMD 之间的转录差异，作为进一步研究区分诊断生物标志物的基础。我们对分别从患有 CETL 和 IMD 的狗的福尔马林固定和石蜡包埋的皮肤活检中提取的 RNA 进行了 100bp 单端测序。 DESeq2用于主成分分析（PCA）和差异基因表达分析。使用两种不同的工具分析表达显着不同的基因的富集途径。通过 RT-qPCR 和免疫组织化学验证所选基因及其蛋白质的表达。 PCA 证明了 CETL 和 IMD 的不同基因表达谱。与 IMD 相比，CETL 中总共有 503 个基因上调，而 4986 个基因下调。 RT-qPCR证实了5/6所选测试基因的测序结果，而免疫组化检测到的蛋白表达并不完全一致。我们的研究揭示了犬 CETL 和 IMD 之间的转录差异，与人类皮肤淋巴瘤相似。差异表达基因是潜在的歧视性标记，但需要在更大的样本集合上进一步验证。