Abstract

The chemical profiling of the main phytoconstituents of total ethanolic extract (TEE) and its different fractions of Bignonia binata leaves was dereplicated using liquid chromatography–high resolution-electrospray ionisation–mass spectrometry (LC-HR-ESI-MS), revealed the presence of various classes of secondary metabolites; eight phenylethanoids, two flavonoidal glycosides and two iridoids. Moreover, the hepatoprotective and nephroprotective activities of the TEE and its different fractions were investigated in carbon tetrachloride (CCl₄)-intoxicated rats and were compared with those of silymarin-treated group, revealing the highest potency of the EtOAc group, followed by the aqueous one in improving the CCl₄-induced alterations in several biochemical parameters. Besides, EtOAc and aqueous fractions exhibited the most inhibition of CCl₄-induced inflammatory mediators and improving the changes in the histopathological structures of the liver and kidney. In addition, the EtOAc fraction demonstrated the highest total phenolic content, whereas TEE showed the highest amount of total flavonoid content.

摘要

使用液相色谱 - 高分辨率 - 电喷雾电离 - 质谱法 (LC-HR-ESI-MS)对总乙醇提取物 (TEE) 及其不同部分的Bignonia binata叶的主要植物成分进行化学分析，揭示了各类次生代谢物；八种苯乙醇、两种黄酮苷和两种环烯醚萜。此外，在四氯化碳 (CCl ₄ ) 中毒的大鼠中研究了 TEE 及其不同组分的保肝和肾保护活性，并与水飞蓟素处理组进行了比较，发现 EtOAc 组的效力最高，其次是水一是改进CCl ₄-诱导的几个生化参数的改变。此外，EtOAc和水性组分对CCl ₄诱导的炎症介质的抑制作用最大，并改善了肝脏和肾脏组织病理学结构的变化。此外，EtOAc 馏分表现出最高的总酚含量，而 TEE 表现出最高的总黄酮含量。