BACKGROUND Purple corn (Zea mays L.) is one of the main economic crops in China and has been used in treatment of cystitis, urinary infections and obesity. However, purple corncob, the by-products after its processing, appears intense purple black color and is normally disposed as waste or used as animal feed. Therefore, to further expand the medicinal value of purple corncob, the purification and content of the anthocyanins of purple corncob were carried out, and the effect and mechanism of purified purple corncob anthocyanins (PPCCA) on CCl4 -induced chronic liver injury in mice was investigated. RESULTS It was observed that the total anthocyanin content (TAC) from PPCCA (317.51±9.30 mg C-3-G g-1 DW) was significantly higher than that from the purified purple corn seeds anthocyanin (266.73±3.67 mg C-3-G g-1 DW), of which cyanidin 3-O-glucoside (C-3-G) accounted for 90.6% and 90.4% of the TAC, respectively. Furthermore, compared with the CCl4 group, PPCCA treatment significantly reduced liver index, serum total bilirubin (T-Bil), Alanine transaminase (ALT), aspartate transaminase (AST), and liver malondialdehyde (MDA) levels, but increased liver superoxide dismutase (SOD) activity. The pathological changes were also improved, such as more regular arrangement of hepatocytes, less swelling, vacuoles and apoptosis cells. Additionally, mechanistic studies showed that PPCCA down-regulated the expression of Caspase-3, Bax and cytochrome P450 2E1 (CYP2E1) proteins in the liver and up-regulated the expression of Bcl-2. CONCLUSION These results demonstrated that PPCCA could ameliorate CCl4 -induced chronic liver injury by regulating oxidative stress and hepatocyte apoptosis pathways. This article is protected by copyright. All rights reserved.

中文翻译：

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Zea mays L. cob 纯化花​​青素通过调节氧化应激和细胞凋亡改善小鼠慢性肝损伤

背景技术紫玉米(Zea mays L.)是我国主要的经济作物之一，已被用于治疗膀胱炎、泌尿系统感染和肥胖症。然而，加工后的副产品紫色玉米芯呈深紫黑色，通常作为废物处理或用作动物饲料。因此，为进一步扩大紫玉米芯的药用价值，开展了紫玉米芯花青素的纯化及含量研究，探讨了纯化紫玉米芯花青素（PPCCA）对四氯化碳致小鼠慢性肝损伤的作用及机制。 . 结果 观察到来自 PPCCA 的总花青素含量 (TAC) (317.51±9.30 mg C-3-G g-1 DW) 显着高于来自纯化紫玉米种子的花青素 (266.73±3.67 mg C-3- G g-1 DW), 其中花青素3-O-葡萄糖苷(C-3-G)分别占TAC的90.6%和90.4%。此外，与CCl4组相比，PPCCA治疗显着降低肝脏指数、血清总胆红素（T-Bil）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和肝脏丙二醛（MDA）水平，但增加肝脏超氧化物歧化酶（ SOD) 活动。病理改变也得到改善，如肝细胞排列更规则，肿胀、空泡和凋亡细胞减少。此外，机制研究表明 PPCCA 下调肝脏中 Caspase-3、Bax 和细胞色素 P450 2E1 (CYP2E1) 蛋白的表达并上调 Bcl-2 的表达。结论 这些结果表明，PPCCA 可以通过调节氧化应激和肝细胞凋亡途径来改善 CCl4 诱导的慢性肝损伤。本文受版权保护。版权所有。