A bacterium designated as strain STP14 was isolated from a sewage treatment plant and identified as Acinetobacter calcoaceticus based on 16S ribosomal RNA gene sequencing. Strain STP14 exhibited resistance to several metals such as mercury, cobalt, copper, nickel, lead, and cadmium. Among these metals, the bacterium showed maximum resistance to cadmium in concentration up to 1200 mg/L. The antimicrobial susceptibility test of A. calcoaceticus strain STP14 showed coresistance to all tested antibiotics except tigecycline and chloramphenicol for which 16 ± 1- and 15 ± 1-mm zone of inhibition was observed, respectively. The protein pattern of the crude cellular extract revealed substantial differences in protein bands of untreated control and cadmium treated A. calcoaceticus strain STP14 suggesting variable protein expression under cadmium stress. Metals and antibiotic resistance are increasing phenomenon and universal concern of public health. This study improves our understanding regarding the bacterial coresistance against metals and antibiotics and the possible emergence of multidrug resistance due to selective pressure and coselection in the metal polluted sewage sludge.

中文翻译：

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污水处理厂报告的耐镉和抗生素的醋酸钙不动杆菌菌株 STP14

从污水处理厂中分离出一种名为 STP14 菌株的细菌，根据 16S 核糖体 RNA 基因测序鉴定为醋酸钙不动杆菌。菌株 STP14 表现出对几种金属的耐受性，例如汞、钴、铜、镍、铅和镉。在这些金属中，细菌对浓度高达 1200 mg/L 的镉表现出最大的抵抗力。醋酸钙菌株 STP14 的抗微生物药敏试验表明，除替加环素和氯霉素外，对所有测试的抗生素均具有共抗性，分别观察到 16 ± 1- 和 15 ± 1-mm 的抑菌圈。粗细胞提取物的蛋白质模式显示未处理的对照和镉处理的 A.calcoacetus 菌株 STP14 的蛋白质条带存在显着差异，表明在镉胁迫下蛋白质表达可变。金属和抗生素耐药性是日益增多的现象和公众健康普遍关注的问题。这项研究提高了我们对细菌对金属和抗生素的共抗性以及由于金属污染的污水污泥中的选择压力和共选择而可能出现的多药耐药性的理解。