Anammox biotechnology has been widely applied for its attractive advantages, but its application has been seriously limited due to the instinctive drawback of nitrate production. In this work, a novel Sequential Anammox and Denitrification (SAD) system was developed for the advanced nitrogen removal by using solid carbon source (SCS) and coupling anammox with denitrification. The long-term operation results demonstrated that the SAD system could remove the total nitrogen (TN) efficiently, with the effluent TN concentration of 1.4 ± 0.5 mg N/L, the TN removal efficiency (NRE) of 99.3 ± 0.2%, and the TN removal rate (NRR) of 1.7 ± 0.1 kg/(m³·d). The determination results showed that SCS had a good property for sustained release of COD, with a dissolved organic yield (by COD) of 1.1 g-COD/g-rice. When the addition rate was set at 6 g-rice/7-days, the COD release rate of 0.9 kg-COD/(m³·d) from SCS matched the nitrate production rate of 1.2 × 10⁻¹ kg-N/(m³·d) from anammox with consumption ratio of 7.5. The analysis on the microbial community revealed that Candidatus_Brocadia and Denitratisoma were the dominant functional bacteria for anammox and denitrification, which contributed to about 92.7% and 6.6% of the total nitrogen removal, respectively. This work is helpful for the innovation and application of anammox-based technology.

厌氧氨氧化生物技术因其吸引人的优势而被广泛应用，但由于硝酸盐生产的本能缺陷，其应用受到了严重限制。在这项工作中，开发了一种新型的顺序厌氧和反硝化（SAD）系统，该系统通过使用固体碳源（SCS）并将厌氧氨氧化与反硝化耦合来进行高级脱氮。长期运行结果表明，SAD系统可有效去除总氮（TN），出水TN浓度为1.4±0.5 mg N / L，TN去除效率（NRE）为99.3±0.2％， TN去除率（NRR）为1.7±0.1 kg /（m ³·d）。测定结果表明，SCS具有良好的COD持续释放性能，其溶解有机物产量（按COD计算）为1.1 g-COD / g-大米。当添加速率设置为6 g-大米/ 7天时，来自SCS的COD释放速率为0.9 kg-COD /（m ³ ·d）与硝酸盐产生速率为1.2×10 ^-1  kg-N /（ m ³ ·d）来自厌氧氨水，消耗率为7.5。上的微生物群落分析表明，暂定_Brocadia和Denitratisoma为优势功能菌为厌氧氨氧化和反硝化，这有助于约92.7％，总脱氮的6.6％。这项工作有助于基于厌氧氨氧化技术的创新和应用。