Black soldier fly larvae meal (BSFLM) has been successfully demonstrated as a promising fish meal (FM) replacer in diets of several fish species including European seabass (Dicentrarchus labrax). However, its impacts on antioxidant capacity, and immune responses of treated fish are still poorly understood. A 60-day feeding trial was carried out to evaluate the effects of partial substitution of FM with different levels of dry BSFLM on the antioxidative status, non-specific immunity, transcriptomic responses, and resistance of European seabass to the challenge with Vibrio alginolyticus. Four isoproteic (45%) and isolipidic diets were formulated by replacing 0.0%, 25%, 35%, and 50% of the dietary FM. Each diet was randomly assigned to four fish groups (in triplicates) (initial mean body weight, 12.1 ± 0.21 g) (20 fish per aquarium) (n = 240). Fish were fed three times daily to the apparent satiation. At the end of the feeding trial, serum antioxidant biomarkers such as malondialdehyde levels, and catalase, superoxide dismutase, and glutathione peroxidase enzyme activities were significantly increased in all BSFLM groups in a dose-dependent manner compared to the control group (P < 0.05). The non-specific immune indices, including phagocytic activity, phagocytic index, serum lysozyme and respiratory burst activities were significantly elevated in BSFLM groups compared to those in the control group (P < 0.05). Significant upregulation of the mRNA expression levels of hepatic heat shock protein 70, interleukin-1beta and interleukin-10 genes were observed in all BSFLM groups compared to the control group (P < 0.05). Additionally, after the challenge with V. alginolyticus, the relative percent of survival was significantly elevated in fish groups fed on diets containing graded levels of BSFLM over the control group (P < 0.05). Conclusively, the present study suggests the potential efficacy of partial replacement of dietary FM protein for up to 50% by BSFLM without negative effects on fish health with possible potentiation of the antioxidative status, and the immune responses of the European seabass.

黑士兵蝇fly粉已被成功证明是一种有前途的鱼粉（FM）替代品，可用于包括欧洲鲈鱼（Dicentrarchus labrax）在内的几种鱼类的日粮中。然而，其对抗氧化能力的影响以及处理鱼的免疫应答仍知之甚少。进行了为期60天的喂养试验，以评估以不同水平的干BSFLM部分替代FM对欧洲海鲈抗藻溶藻弧菌的抗氧化状态，非特异性免疫，转录组反应和抗性的影响。通过替代饮食中FM的0.0％，25％，35％和50％，配制了四种等蛋白饮食（45％）和等脂饮食。每种饮食被随机分为四组（一式三份）（初始平均体重为12.1±0.21 g）（每个水族馆20条鱼）（n  = 240）。每天给鱼喂3次明显的饱食。在喂养试验结束时，与对照组相比，所有BSFLM组的血清抗氧化剂生物标志物（如丙二醛水平，过氧化氢酶，超氧化物歧化酶和谷胱甘肽过氧化物酶的活性）均呈剂量依赖性显着增加（P <0.05）。BSFLM组的非特异性免疫指数，包括吞噬活性，吞噬指数，血清溶菌酶和呼吸爆发活性，均明显高于对照组（P  <0.05）。与对照组相比，所有BSFLM组均观察到肝热休克蛋白70，白介素-1β和白介素10基因的mRNA表达水平显着上调（P  <0.05）。此外，在用溶藻弧菌攻击后，饲喂含分级BSFLM水平日粮的鱼组的相对存活率显着高于对照组（P <0.05）。总之，本研究表明，用BSFLM替代50％的膳食FM蛋白具有潜在的功效，而对鱼类健康没有负面影响，并可能增强抗氧化状态以及欧洲鲈鱼的免疫反应。