To select potato genotypes tolerant to water deficit, systems to simulate this stress have been used. Polyethylene glycol (PEG) is the main osmotic agent used for this purpose, but it causes an excessively severe stress. However, it is difficult to carry out an experiment that aims to compare plant responses under water deficit by osmotic or matric induction, and, thus, few studies compare these stress-inducing mechanisms. Therefore, the objective of this study was to compare the responses of Agata, BRS Clara, C2406-03 and Cota genotypes to water deficit in both induction methods (matric or osmotic). The tests were carried out in a greenhouse, one using hydroponics (osmotic induction) and the other in pots with soil (matric induction). In both tests, the application of stressful conditions occurred at the beginning of tuberization. Assessments of gas exchange and shoot temperature were made throughout the exposure to stress. Also, samples were collected from leaves for analysis of osmotic potential and leaves and tubers for analysis of metabolite content. At the end of the potato plant cycle, the number and weight of tubers were evaluated. In both stress conditions, there were significant reductions in photosynthesis and transpiration rate compared to the respective normal hydration conditions. In addition, indicators such as metabolite levels (proline and soluble sugars) were significantly altered in plants exposed to different stress inductions. These data, together with the significant limitations in the growth of stressed plants, indicate that the experimental models induce similar responses. However, the water deficit by osmotic induction was more severe for the potato plants when compared to stress due to matric induction, mainly affecting tuber production. Therefore, the water deficit osmotic induction model can be recommended for phenotyping tolerance to this stress, due to the hydroponic system inducing greater tuber production per plant under optimal cultivation conditions.

为了选择耐缺水的马铃薯基因型，已经使用了模拟这种胁迫的系统。聚乙二醇（PEG）是用于此目的的主要渗透剂，但会引起过大的压力。然而，难以进行旨在通过渗透或基质诱导来比较缺水下的植物响应的实验，因此，很少有研究比较这些胁迫诱导机理。因此，本研究的目的是比较两种诱导方法（基质或渗透法）中Agata，BRS Clara，C2406-03和Cota基因型对缺水的反应。测试是在温室中进行的，一种使用水培法（渗透诱导），另一种使用土壤的盆（矩阵诱导）。在这两个测试中，在块茎开始时就施加了压力条件。在整个应力暴露过程中进行气体交换和射流温度的评估。同样，从叶片中收集样品用于分析渗透势，从叶片和块茎中收集样品用于分析代谢物含量。在马铃薯种植周期结束时，评估了块茎的数量和重量。与相应的正常水合条件相比，在两种胁迫条件下，光合作用和蒸腾速率均显着降低。此外，暴露于不同胁迫诱导的植物中的代谢物水平（脯氨酸和可溶性糖）等指标发生了显着变化。这些数据，再加上胁迫植物生长的显着局限性，表明实验模型诱导出相似的反应。然而，与基质诱导的胁迫相比，渗透诱导的马铃薯植株水分亏缺更为严重，主要影响块茎生产。因此，由于水培系统在最佳栽培条件下可诱导每株植物更大的块茎产量，因此可以推荐水分亏缺渗透诱导模型来应对这种胁迫的表型耐受性。