The use of probiotics can beneficially improve the water quality in the biofloc technology (BFT) system consequently enhancing the fish performance. This study focused on the effect of Bacillus subtilis on a clear water static system compared to the BFT system on the productive performance, proximate body composition, and diversity of intestinal bacterial communities of tambaqui (Colossoma macropomum) juveniles submitted to four treatments: clear water static system with (CW + BS) or without (CW) B. subtilis and BFT with (BFT + BS) or without (BFT) B. subtilis. For the study, 20 juveniles (five replicates) per treatment were used for 60 days. For the B. subtilis treatments, water was inoculated at weekly intervals with a 4 × 10⁸-cell mL⁻¹ culture suspension. Results revealed that regardless of B. subtilis supplementation, fish reared in BFT displayed better zootechnical performance than those in CW—weight gain (BFT 33.57 ± 4.08 g; CW 19.97 ± 5.42 g), protein efficiency (BFT 0.16 ± 0.02; CW 0.11 ± 0.02), feed conversion ratio (BFT 0.71 ± 0.08; CW 0.84 ± 0.40), and relative growth rate (BFT 1.52 ± 0.12; CW 1.06 ± 0.15)—which suggests that biofloc consumption was 31.9% crude protein. Moreover, fish reared in BFT + BS had a higher condition factor (2.30 ± 0.09). In conclusion, BFT is regarded as a promising system to save water, decrease aquaculture effluents, and promote tambaqui farming. Although B. subtilis is a common bacterium found in tambaqui gut, its supplementation in the BFT system improved the fish condition factor.

益生菌的使用可以有益地改善生物絮凝技术（BFT）系统中的水质，从而提高鱼类的性能。这项研究的重点是与BFT系统相比，枯草芽孢杆菌对透明水静态系统的影响对提交四种处理的坦巴基（Colossoma macropomum）幼鱼的生产性能，邻近身体组成和肠道细菌群落多样性的影响带（CW + BS）或不带（CW）枯草芽孢杆菌的系统和带（BFT + BS）或不带（BFT）枯草芽孢杆菌的BFT系统。在研究中，每次治疗使用20个少年（5个重复样本），共60天。对于枯草芽孢杆菌在处理过程中，每周用4×10 ⁸细胞mL ^-1培养悬浮液接种水。结果表明，与补充枯草芽孢杆菌无关，在BFT中饲养的鱼表现出比CW更好的动物技术性能-增重（BFT 33.57±4.08 g； CW 19.97±5.42 g），蛋白质效率（BFT 0.16±0.02； CW 0.11± 0.02），饲料转化率（BFT 0.71±0.08; CW 0.84±0.40）和相对生长速率（BFT 1.52±0.12; CW 1.06±0.15）—这表明生物絮凝剂消耗量为31.9％的粗蛋白。此外，在BFT + BS中饲养的鱼的条件因子较高（2.30±0.09）。总之，BFT被认为是节约用水，减少水产养殖废水，促进坦巴基养殖的有前途的系统。虽然枯草芽孢杆菌是坦巴基肠中常见的细菌，其在BFT系统中的补充改善了鱼类的状况。