The rapid spread of antibiotic resistance has increased research interest in the discovery of natural products, mainly from actinomycetes, which have been the primary source of antimicrobial compounds. This study aimed to isolate, characterize, and optimize the production of some of the bioactive compounds from bioactive soil actinomycetes. One promising soil actinomycete, which was molecularly identified as Streptomyces sp. and designated as Streptomyces sp. MS. 10, showed broad-spectrum antimicrobial activity, including activity against methicillin-resistant Staphylococcus aureus. Thus, it was selected for isolation of its major bioactive compounds. Polymerase chain reaction amplification of the genes responsible for antibiotic biosynthesis showed the presence of genes encoding type I and type II polyketide synthase. Liquid chromatography-mass spectrometry analysis found that the major antimicrobial compounds produced by Streptomyces sp. MS. 10 were weakly ionized bioactive secondary metabolites. A large-scale fermentation experiment of Streptomyces sp. MS. 10 using pre-optimized culture conditions followed by bioassay-guided chromatographic separation of its secondary metabolites resulted in the isolation of putative bioactive compounds that were identified as fatty acids using proton nuclear magnetic resonance spectroscopy. Egyptian soil is still a good source for exploring bioactive actinomycetes. Additionally, this study highlighted the importance of combining both physicochemical and genotypic characterization with spectroscopic analysis of the major natural products when isolating bioactive metabolites.

中文翻译：

---

从埃及土壤分离株 Streptomyces sp. 中分离和优化生产推定的抗菌化合物。小姐。10

抗生素耐药性的迅速蔓延增加了对天然产物发现的研究兴趣，主要来自放线菌，放线菌是抗菌化合物的主要来源。本研究旨在从生物活性土壤放线菌中分离、表征和优化一些生物活性化合物的生产。一种有前途的土壤放线菌，在分子上被鉴定为链霉菌属。并指定为链霉菌属。小姐。10，显示出广谱抗菌活性，包括对耐甲氧西林金黄色葡萄球菌的活性。因此，它被选择用于分离其主要生物活性化合物。负责抗生素生物合成的基因的聚合酶链反应扩增显示存在编码 I 型和 II 型聚酮化合物合酶的基因。液相色谱-质谱分析发现链霉菌产生的主要抗菌化合物。小姐。10 个是弱电离的生物活性次级代谢物。链霉菌属的大规模发酵实验。小姐。10 使用预先优化的培养条件，然后对其次生代谢物进行生物测定引导的色谱分离，从而分离出推定的生物活性化合物，这些化合物使用质子核磁共振波谱法鉴定为脂肪酸。埃及土壤仍然是探索生物活性放线菌的良好来源。此外，这项研究强调了在分离生物活性代谢物时将物理化学和基因型表征与主要天然产物的光谱分析相结合的重要性。