Novel Strategy of Mussel-Inspired Immobilization of Naringinase with High Activity Using a Polyethylenimine/Dopamine Co-deposition Method,ACS Omega

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Novel Strategy of Mussel-Inspired Immobilization of Naringinase with High Activity Using a Polyethylenimine/Dopamine Co-deposition Method
ACS Omega ( IF 3.7 ) Pub Date : 2021-01-22 , DOI: 10.1021/acsomega.0c05756
Xinyu Zheng ₁ , Qian Li ₁ , Jing Tian ₁ , Honglei Zhan ₁ , Chan Yu ₁ , Shujing Wang ₁ , Xitong Sun ₂

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Mussel-inspired surface chemistry is recognized as a simple, efficient, and mild surface modification method and has become a research hotspot in many fields. In this study, polyethylenimine/dopamine was coated on the surface of SBA-15 using a co-deposition method, making it possible to immobilize naringinase with high activity and operation stability. The optimal modification and immobilization conditions as well as enzyme properties were investigated. The naringinase activity can reach up to 753.78 U/g carrier, which was much higher than those of the previous works. Besides, the residual naringinase activity still kept 78.91% of the initial activity after one month of storage and maintained 60.79% after 8 cycles. Therefore, the strategy of mussel-inspired enzyme immobilization could be recognized as a promising and universal enzyme immobilization method, with the advantages of high relative enzyme activity, enzyme carrying rate, enzyme activity recovery rate, and good reusability and storage stability.

中文翻译：

采用聚乙烯亚胺/多巴胺共沉积法仿贻贝固定高活性柚皮苷酶的新策略

贻贝表面化学被认为是一种简单、高效、温和的表面改性方法，已成为许多领域的研究热点。本研究采用共沉积方法将聚乙烯亚胺/多巴胺涂覆在SBA-15表面，使得固定化具有高活性和运行稳定性的柚皮苷酶成为可能。研究了最佳修饰和固定化条件以及酶的性质。柚皮苷酶活性可达753.78 U/g载体，远高于以往的工作。此外，保存1个月后，残余柚皮苷酶活性仍保持初始活性的78.91%，8个循环后仍保持60.79%。因此，贻贝酶固定化策略被认为是一种有前途且通用的酶固定化方法，具有较高的相对酶活性、酶携带率、酶活性回收率以及良好的重复使用性和储存稳定性等优点。

更新日期：2021-02-02

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