The loss of redox homeostasis induced by hyperglycemia is an early sign and key factor in the development of diabetic retinopathy. Due to the high level of long-chain polyunsaturated fatty acids, diabetic retina is highly susceptible to lipid peroxidation, source of pathophysiological alterations in diabetic retinopathy. Previous studies have shown that pterostilbene, a natural antioxidant polyphenol, is an effective therapy against diabetic retinopathy development, although its protective effects on lipid peroxidation are not well known. Plasma, urine and retinas from diabetic rabbits, control and diabetic rabbits treated daily with pterostilbene were analyzed. Lipid peroxidation was evaluated through the determination of derivatives from arachidonic, adrenic and docosahexaenoic acids by ultra-performance liquid chromatography coupled with tandem mass spectrometry. Diabetes increased lipid peroxidation in retina, plasma and urine samples and pterostilbene treatment restored control values, showing its ability to prevent early and main alterations in the development of diabetic retinopathy. Through our study, we are able to propose the use of a derivative of adrenic acid, 17(RS)-10-epi-SC-Δ¹⁵-11-dihomo-IsoF, for the first time, as a suitable biomarker of diabetic retinopathy in plasmas or urine.

中文翻译：

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UPLC-MS / MS分析脂质过氧化作用和天然多酚紫檀的视黄醛保护作用

高血糖引起的氧化还原稳态的丧失是糖尿病性视网膜病发展的早期迹象和关键因素。由于高含量的长链多不饱和脂肪酸，糖尿病视网膜对脂质过氧化反应高度敏感，脂质过氧化反应是糖尿病性视网膜病中病理生理改变的来源。先前的研究表明，尽管天然抗氧化剂多酚紫檀对糖尿病性视网膜病的发展有效，但它对脂质过氧化的保护作用尚不清楚。分析了每天用翼戊二烯治疗的糖尿病兔，对照组和糖尿病兔的血浆，尿液和视网膜。通过确定花生四烯酸的衍生物评估脂质过氧化作用，高效液相色谱-串联质谱联用测定肾上腺素和二十二碳六烯酸。糖尿病会增加视网膜，血浆和尿液样本中脂质的过氧化作用，而蝶芪治疗可恢复控制值，显示其具有预防糖尿病性视网膜病发展的早期和主要改变的能力。通过我们的研究，我们可以建议使用肾上腺酸17（RS）-10-外延- SC -Δ ¹⁵ -11-二高-γ-ISOF，对于第一次，如糖尿病性视网膜病的在等离子体中或尿合适的生物标志物。