Although lncRNAs have been identified as playing critical roles in the development of germ cells, their potential involvement in the development of PGCs in chickens remains poorly understood. Differentially expressed lncRNAs (DELs) from previous RNA-seq of embryonic stem cells (ESCs), PGCs, and spermatogonial stem cells (SSCs) were analyzed by K-means clustering, from which a key candidate, lncRNA (lncRNA PGC regulator, LncPGCR) was obtained. We confirmed that LncPGCR plays a positive role in the development of PGCs by increasing the expression of the PGC marker gene (Cvh and C-kit), while downregulating the pluripotency-associated gene (Nanog) in vitro and in vivo. The activation and expression of LncPGCR are regulated by histone acetylation, and transcription factor TCF7L2. Mechanistically, a rescue assay was performed to further confirm that LncPGCR contributed to the development of PGCs by regulating the gga-miR-6577-5p/Btrc signaling pathway. Adsorption of gga-miR-6577-5p activated the WNT signaling cascade by relieving the gga-miR-6577-5p-dependent inhibition of Btrc expression. Taken together, our study discovered the growth-expedited role of LncPGCR in PGCs development, showing the potential LncPGCR/miR-6577-5p/Btrc pathway. The results and findings provide a novel insight into the development of PGCs.

中文翻译：

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TCF7L2介导的长非编码RNA LncPGCR调节鸡的原始生殖细胞形成。

尽管已确认lncRNA在生殖细胞的发育中起关键作用，但它们对鸡PGC发育的潜在参与仍知之甚少。通过K-均值聚类分析了与胚胎干细胞（ESC），PGC和精原干细胞（SSC）的先前RNA-seq差异表达的lncRNA（DELs），然后从中筛选出关键候选lncRNA（lncRNA PGC调节剂LncPGCR）。获得了。我们证实LncPGCR通过增加PGC标记基因（Cvh和C-kit）的表达，同时在体内外下调多能性相关基因（Nanog），在PGC的发展中发挥积极作用。的激活和表达LncPGCR受组蛋白乙酰化和转录因子TCF7L2调控。从机制上讲，进行了拯救试验以进一步证实LncPGCR通过调节gga-miR-6577-5p / Btrc信号通路来促进PGC的发展。gga-miR-6577-5p的吸附通过减轻bgrc表达的gga-miR-6577-5p依赖性抑制作用激活了WNT信号级联。综上所述，我们的研究发现LncPGCR在PGC的发育中具有促进生长的作用，显示了潜在的LncPGCR / miR-6577-5p / Btrc途径。结果和发现为PGC的发展提供了新颖的见解。