High‐efficiency saccharification technology is one of the bottlenecks of cellulosic bio‐hydrogen production. Cellulosic feedstocks saccharification currently performed by commercial cellulase, which is composed of different fungal cellulase. Compared with fungi, thermocellulosic bacteria represented by Ruminiclostridium thermocellum have a complete cellulase system, and a higher cellulase catalytic efficiency than fungi; however, R. thermocellum is susceptible to feedback inhibition by cellobiose, which limits the application of R. thermocellum on cellulosic bio‐hydrogen production. In this study, a strain named R. thermocellum M3, which is not subject to feedback inhibition by cellobiose, was used in the bio‐hydrogen production of cellulosic agricultural waste feedstocks to explore the feasibility of bacterial saccharification of cellulosic substrates for biological hydrogen production.

中文翻译：

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用Ruminiclostridium thermocellum M3进行生物强化，以提高农业固体废物产生的嗜热氢

高效糖化技术是纤维素生物制氢的瓶颈之一。纤维素原料的糖化目前是由商业纤维素酶进行的，其由不同的真菌纤维素酶组成。与真菌相比，以热纤梭菌为代表的热纤维素细菌具有完整的纤维素酶系统，并且比真菌具有更高的纤维素酶催化效率。然而，热纤菌很容易受到纤维二糖的反馈抑制，这限制了热纤菌在纤维素生物制氢中的应用。在这项研究中，一个名为R. thermocellum的菌株 M3不受纤维二糖的反馈抑制，被用于纤维素农业废料原料的生物制氢，以探索将纤维素底物进行细菌糖化以生产生物制氢的可行性。