Syrian hamsters (Mesocricetus auratus) are largely used as a model for infectious diseases because it is very susceptible to several pathogens, including Leishmania spp. parasites. However, the research community faces limitations in its use due to the lack of immunological reagents and tools to study the immune system in this model. In this context, we proposed the validation of some important commercially anti‐mouse mAbs (CD4, TNF‐α, IFN‐γ and IL‐10) and how this could be useful to evaluate a specific cellular immune response in Leishmania‐infected hamster using flow cytometry experiments. Our data demonstrated a cross‐reactivity between these anti‐mouse mAbs and hamster molecules that were herein studied. Beyond that, it was able to characterize the development of a specific cellular immune response through cytokine production in L infantum‐infected hamsters when compared to uninfected ones. These data not only aid the usage of hamsters as experimental model to investigate various infectious diseases, but they contribute to the design of novel approaches to further investigate the immunological mechanisms associated to pathogen infections.

中文翻译：

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单克隆抗体的建立以评估L婴儿感染仓鼠模型中的细胞免疫

叙利亚仓鼠 ( Mesocricetus auratus ) 主要用作传染病模型，因为它对多种病原体非常敏感，包括利什曼原虫。寄生虫。然而，由于缺乏用于研究该模型中免疫系统的免疫试剂和工具，研究界在其使用方面面临限制。在这种情况下，我们提议验证一些重要的商业抗小鼠 mAb（CD4、TNF-α、IFN-γ 和 IL-10）以及这如何有助于评估利什曼原虫的特定细胞免疫反应- 使用流式细胞术实验感染的仓鼠。我们的数据证明了这些抗小鼠 mAb 与本文研究的仓鼠分子之间的交叉反应性。除此之外，与未感染的仓鼠相比，它能够通过在L 婴儿感染的仓鼠中产生细胞因子来表征特定细胞免疫反应的发展。这些数据不仅有助于将仓鼠用作研究各种传染病的实验模型，而且有助于设计新方法以进一步研究与病原体感染相关的免疫机制。