Hepatocellular carcinoma (HCC) is one of the most common cancers with high prevalence and mortality, and it has brought huge economic and health burden for the world. It is urgent to found novel targets for HCC diagnosis and clinical intervention. Circular RNA (circRNA) has been reported to participate in many cancer progressions including HCC, suggesting that circRNA might paly essential role in HCC initiation and progression. Our study aims to found that potential circRNA participates in HCC development and its underlying molecular mechanisms. We obtained three pairs of HCC tissues and its adjacent normal tissues data from GEO DataSets. MTT, cell colony, EdU, wound‐healing, transwell invasion and mouse xenograft model assays were used to demonstrate the biological functions of circCAMSAP1 in HCC progression. Furthermore, we conducted bioinformatics analysis, AGO2‐RIP, RNA pull‐down and luciferase reporter assays to assess the association of circCAMSAP1‐miR‐1294‐GRAMD1A axis in HCC cells. The expression of circCAMSAP1 was up‐regulated in HCC tissues compared with its adjacent normal tissues. Up‐regulation of circCAMSAP1 promoted HCC biological functions both in vitro and in vivo. The promotive effects of circCAMSAP1 on HCC progression function through miR‐1294/GRAMD1A pathway. CircCAMSAP1 was up‐regulated in HCC tissues, and circCAMSAP1 up‐regulated GRAMD1A expression to promote HCC proliferation, migration and invasion through miR‐1294. CircCAMSAP1 might be a potential prognosis and therapeutic target for HCC.

中文翻译：

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CircCAMSAP1通过miR-1294/GRAMD1A通路促进肝细胞癌进展

肝细胞癌（HCC）是最常见的癌症之一，发病率和死亡率都很高，给世界带来了巨大的经济和健康负担。迫切需要为 HCC 诊断和临床干预寻找新的靶点。据报道，环状 RNA (circRNA) 参与了包括 HCC 在内的许多癌症进展，这表明 circRNA 可能在 HCC 的发生和进展中发挥重要作用。我们的研究旨在发现潜在的 circRNA 参与 HCC 的发展及其潜在的分子机制。我们从 GEO 数据集中获得了三对 HCC 组织及其邻近的正常组织数据。MTT、细胞集落、EdU、伤口愈合、transwell 侵袭和小鼠异种移植模型测定用于证明circCAMSAP1的生物学功能在 HCC 进展中。此外，我们进行了生物信息学分析、AGO2-RIP、RNA 下拉和荧光素酶报告基因检测，以评估circCAMSAP1-miR-1294-GRAMD1A轴在 HCC 细胞中的关联。与邻近的正常组织相比，circCAMSAP1在HCC组织中的表达上调。上调的circCAMSAP1在体外和体内促进肝癌的生物学功能。circCAMSAP1通过miR-1294/GRAMD1A通路对HCC进展功能的促进作用。CircCAMSAP1在 HCC 组织中上调，circCAMSAP1上调GRAMD1A表达通过miR-1294促进 HCC 增殖、迁移和侵袭。CircCAMSAP1可能是 HCC 的潜在预后和治疗靶点。