In order to prevent thrombosis, reduce intima hyperplasia, and to maintain long-term patency after implantation of an artificial blood vessel, the formation of intact endothelial cells layer on an inner surface of graft is desirable. The present study aimed to improve endothelial cell adhesion by regulating the morphology of the inner surface of artificial blood vessels. Zein fibre membranes with three fibre diameters (small, ~ 100 nm; medium, ~ 500 nm; and large, ~ 1000 nm) were constructed by electrospinning. A flow chamber device was designed to simulate the blood flow environment. The morphology and adhesion of human umbilical vein fusion cells (EAhy926) on the surface of the fibre membranes were studied under a shear stress of approximately 15 dynes/cm². The results showed that oriented electrospun zein fibre surfaces with both medium- and large-diameter fibres can regulate the morphology of endothelial cells (EAhy926), which are aligned by the fibre direction. The three fibre membranes improved the adhesion of endothelial cells significantly compared to that on the flat membrane. When the fibre direction was fixed parallel to the fluid direction, the medium-diameter oriented-fibre membrane could significantly improve the ability endothelial cells to resist shear stress, and there was a significant difference at 1, 2 and 4 h time points compared with the shear stress resistance on the small-diameter and large-diameter oriented-fibre membranes. When the fibre direction was perpendicular to the fluid direction, again the medium-diameter oriented-fibre membrane improved the ability of endothelial cells to resist shear stress significantly at 1 and 2 h time points. It was concluded that by changing the diameter and arrangement of electrospun fibres, cell morphology control and shear stress resistance can be achieved.

为了防止血栓形成，减少内膜增生，并保持人工血管植入后的长期通畅，在移植物的内表面形成完整的内皮细胞层是可取的。本研究旨在通过调节人造血管内表面的形态来改善内皮细胞的粘附。通过静电纺丝构建具有三种纤维直径（小，约 100 nm；中，约 500 nm；和大，约 1000 nm）的玉米蛋白纤维膜。设计了一个流动室装置来模拟血流环境。^{在约15 dynes/cm 2}的剪切应力下研究了人脐静脉融合细胞（EAhy926）在纤维膜表面的形态和粘附. 结果表明，具有中等和大直径纤维的定向电纺玉米醇溶蛋白纤维表面可以调节内皮细胞（EAhy926）的形态，这些细胞按纤维方向排列。与平膜相比，三种纤维膜显着改善了内皮细胞的粘附。当纤维方向与流体方向平行固定时，中径取向纤维膜可显着提高内皮细胞抵抗剪切应力的能力，且在1、2、4 h时间点与对照组相比有显着差异。小直径和大直径定向纤维膜的抗剪应力。当纤维方向垂直于流体方向时，在 1 小时和 2 小时时间点，中径定向纤维膜再次显着提高了内皮细胞抵抗剪切应力的能力。得出的结论是，通过改变电纺纤维的直径和排列，可以实现细胞形态控制和抗剪切应力。