In the framework of the Plastic Busters MPAs project, a harmonization exercise on two methods of microplastic extraction from biological samples i.e. 15% H₂O₂ digestion and 10% KOH digestion was carried out. The two methods were tested in four laboratories on fish gastrointestinal tracts and mussel tissues spiked with polyethylene, polypropylene and polyethylene terephthalate. The recovery percentage of microplastics for each method, species and polymer tested were overall similar among laboratories, and interlaboratory coefficient of variation was less than 11% for the majority of samples. Microplastic recovery rates for the two methods were similar for each sample tested, but overall mean interlaboratory recovery rate using KOH (96.67%) was higher than H₂O₂ (88.75%)_. Results validate the use of both methods for extracting microplastics from biota tissues. However, when comparing the two methods in terms of microplastic recovery rate, time consumed, technical difficulties and cost, digestion with 10% KOH is considered optimal.

在“塑料克星” MPA项目的框架内，对从生物样品中提取微塑料的两种方法进行了协调演练，即15％H ₂ O ₂消化和10％KOH消化。两种方法在四个实验室的鱼肠胃道和贻贝组织中掺有聚乙烯，聚丙烯和聚对苯二甲酸乙二酯的实验室中进行了测试。在实验室之间，每种方法，种类和所测试的聚合物的微塑料回收率总体上相似，并且大多数样品的实验室间变异系数小于11％。两种方法的微塑性回收率对于每个测试样品相似，但使用KOH的整体实验室间平均回收率（96.67％）高于H ₂ O₂（88.75％）_。结果证实了两种方法都可用于从生物体组织中提取微塑料。但是，当从微生物回收率，消耗的时间，技术难度和成本方面比较这两种方法时，认为以10％KOH消解是最佳方法。