In a few recent studies, the action of a bacterial dioxygenase (10S-DOX) on palmitoleic acid was observed within some polar and estuarine settings. To add further mechanistic information regarding the action of this enzyme in marine settings, we measured a range of lipids (sterols, fatty acids and the chlorophyll phytyl side chain) and their biotic and abiotic degradation products in water samples collected in 2018 from two depths (5 m and 25 m) at the temperate oceanographic time series site L4, located in the western English Channel. Lipid distributions indicated a dominance of diatoms and copepods during the spring bloom, while a peak in dinoflagellate activity was evident in samples collected from late summer/autumn, both outcomes being consistent with taxonomic data reported previously for the same sampling site and interval. Monitoring of lipid oxidation products characteristic of different degradation pathways showed a relatively weak effect of photo- and autoxidation processes, with these acting mainly on the more reactive lipids (i.e. chlorophyll and polyunsaturated fatty acids). In contrast, monitoring of biotic degradation processes revealed significant quantities of 10S-hydroxyhexadec-8(E)-enoic acid in samples collected at the end of April (reaching 40% of the residual parent palmitoleic acid), attributed to the involvement of bacterial 10-dioxygenase (10S-DOX) activity during the spring bloom. We propose that this enzyme could be utilised by bacteria to detoxify free fatty acids released by wounded diatoms in the presence of copepods

在最近的一些研究中，细菌双加氧酶（10 S在某些极性和河口环境中观察到棕榈油酸的-DOX）。为了增加有关该酶在海洋环境中作用的进一步的机械信息，我们在2018年从两个深度采集的水样中测量了一系列脂质（固醇，脂肪酸和叶绿素植酸侧链）及其生物和非生物降解产物（位于西英吉利海峡的温带海洋时间序列地点L4的5 m和25 m）处。脂类分布表明，在春季开花期间，硅藻和pe足类动物占优势，而夏末/秋季采集的样品中鞭毛藻活动明显，这两个结果与先前报道的相同采样点和间隔的分类数据一致。监测具有不同降解途径特征的脂质氧化产物显示出光和自氧化过程的作用相对较弱，其中这些过程主要作用于反应性更高的脂质（即叶绿素和多不饱和脂肪酸）。相比之下，对生物降解过程的监测显示有104月底收集的样品中的S-羟基十六烷8（E）-烯酸（达到残留的母体棕榈油酸的40％），这归因于春季细菌10-双加氧酶（10 S -DOX）的活性盛开。我们建议这种酶可以被细菌用来解毒在pe足类动物存在下受伤的硅藻释放的游离脂肪酸