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Anatomical adaptations and mycorrhizal morphology of two threatened Eulophia (Orchidaceae) growing in southern Western Ghats, India
Flora ( IF 1.9 ) Pub Date : 2021-01-23 , DOI: 10.1016/j.flora.2021.151773
Ravichandran Koshila Ravi , Srinivasan Sowmiya , Mayakrishnan Balachandar , Thangavelu Muthukumar

The vegetative anatomy of Eulophia spectabilis (Dennst.) Suresh and Eulophia pulchra (Thouars) Lindl., of subfamily Epidendroideae (Orchidaceae), of subtribe Eulophiinae was investigated to bring out the anatomical differences, mycorrhizal morphology and also their adaptation to thrive in stressful xerophytic habitats. Transverse sections of plant parts were freehand sectioned and stained with various staining solutions for anatomical investigation. Stomatal characteristics were examined by maceration technique. The leaves of both Eulophia species shared similar anatomical characteristics including tertracytic stomata on abaxial surface, uniseriate epidermis on both the surfaces, homogenous mesophyll comprising of intercellular spaces and collateral vascular bundles. Fibre bundles are present only in the leaves of E. pulchra. Likewise, the anatomy of leaf-sheath in both the species resembles those of the leaves except for the large air chambers. The pseudobulb of E. pulchra consists of parenchymatous ground tissue containing air chambers, raphides, starch grains, and water-storage cells and collateral vascular bundles. Starch grains are present only in the rhizomes of E. pulchra ground tissues. In roots, the velamen is undifferentiated in E. pulchra, but distinguished into epivelamen and endovelamen in E. spectabilis. Starch grains are present in root pith cells in E. pulchra. The cell dimensions of most plant parts varied significantly (P<0.05) among the Eulophia species. Contrarily there were no significant (P>0.05) differences in orchid mycorrhizal colonization between the Eulophia species. Thus, these ground orchids possessed several anatomical features that enable them to thrive in dry habitats.



中文翻译:

印度西高止山脉南部生长的两种濒危欧蓝藻(兰科)的解剖学适应性和菌根形态

研究了Euplophiinae亚科Eupendroideae(Orchidaceae)的Eulophia spectabilis(Dennst。)Suresh和Eulophia pulchra(Thouars)Lindl。的营养解剖,以揭示其解剖差异,菌根形态以及对菌根的适应性栖息地。徒手切开植物部分的横切面,并用各种染色液染色以进行解剖学研究。用浸软技术检查气孔特征。两叶的叶子该物种具有相似的解剖学特征,包括背面的叔孔气孔,两个表面上的单性表皮,由细胞间空间和侧支血管束组成的均质叶肉。纤维束仅存在于E. pulchra的叶子中同样,除了大的气室以外,这两个物种的叶鞘解剖结构都类似于叶片。E. pulchra的假鳞茎由薄壁组织组成,其中包含气室,杀虫剂,淀粉颗粒,蓄水细胞和附带的血管束。淀粉粒仅存在于E.pulchra地面组织的根茎中。从根本上说,毛孔虫在E. pulchra中没有分化但在肠球菌中分为上膜和内膜。淀粉粒存在于大肠埃希菌的根髓细胞中Eulophia物种中,大多数植物部位的细胞大小差异显着(P <0.05)。相反,在欧拉氏菌属之间,兰花菌根定植没有显着差异(P> 0.05)。因此,这些地面兰花具有几个解剖特征,使它们能够在干燥的栖息地中繁衍生息。

更新日期:2021-02-01
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