Tide pools are rapidly warming environments with low thermal inertia, where organisms are exposed to potentially stressful conditions. This way, tide pools will be among the first and hardest hit environments by climate warming. Studies of thermal stress in situ are rare, but important so that current thermal stress in the wild can be confirmed and serve as reference for the future. This study aims to investigate the seasonal response of stress biomarkers in a common tide pool fish, the blenny Lipophrys trigloides, under natural conditions, in situ. Multiple biomarkers of thermal and oxidative stress were analysed in the tissues of the muscle, visceral mass, gills and brain of L. trigloides, under spring (18.50 ± 0.71 °C) and summer conditions (28.30 ± 1.30 °C), in order to assess tissue-specific seasonal responses to the rapid warming that occurs seasonally in tide pools. It was concluded that L. trigloides seems to be setting in motion relevant thermal stress and antioxidant defences under summer conditions. All tissues analysed were responsive and all biomarkers of thermal and oxidative stress increased in summer conditions, with the exception of SOD, which presented no alterations. The present study offers reference values that will be useful for future comparison during particularly warm summers and in future years as climate warming progresses.

潮汐池是具有低热惯性的迅速变暖的环境，在此环境中，生物体处于潜在的压力条件下。这样，由于气候变暖，潮汐池将成为首批受灾最严重的环境之一。原位热应力的研究很少，但很重要，因此可以确定当前在野外的热应力，并为将来提供参考。本研究旨在探讨一个共同的潮池鱼，粘鱼的应激生物标志物的季节性响应Lipophrys trigloides，在自然条件下，在原地。热稳定性和氧化应激的多种生物标志物的肌肉，内脏团组织进行了分析，鳃和脑L. trigloides在春季（18.50±0.71°C）和夏季（28.30±1.30°C）下，以评估潮汐池中季节性快速变暖的组织特定季节响应。结论是，在夏季条件下，L。trigloides似乎正在引起运动相关的热应激和抗氧化防御。在夏季条件下，所有分析的组织都具有响应性，并且所有热和氧化应激的生物标志物都增加了，SOD除外，后者无变化。本研究提供参考值，这些参考值将有助于在特别温暖的夏季和未来几年随着气候变暖的进展进行比较。