The development of novel imaging agents for fluorescence detection of latent fingerprint is still important for criminal investigation and identity recognition. Herein, we reported a simple and robust method to prepare multi-color conjugated polymer micelles for visualization of fingerprints. A conjugated polyelectrolyte, poly[(9,9-bis(3′-((N,N-dimethyl)-N-ethylammonium)-propyl)-2,7-fluorene)-alt-2,7-(9,9-dioctylfluorene)] (PFN), was dissolved in water forming polymer micelles with blue emission. Green and red emissions were facilely achieved by incorporating small organic fluorophores including coumarin 6 (C6) and Nile red (NR) into the PFN micelles, respectively. These micelles exhibited bright fluorescence both in solution and solid film, where significant aggregation-caused quenching was avoided and fluorescence resonance energy transfer from PFN to C6 or NR was enhanced. Through a simple solution method, these polymer micelles proved their high affinity towards the fingerprint ridges. Accordingly, fluorescent fingerprint images with high-resolution were obtained, which were less interfered by fluorescence background from substrate and overlap between adjacent fingerprints.

用于潜在指纹荧光检测的新型显像剂的开发对于犯罪调查和身份识别仍然很重要。在这里，我们报道了一种简单而健壮的方法来制备多色共轭聚合物胶束以可视化指纹。共轭聚电解质，聚[（9,9-bis（3'-（（N，N将-（二甲基）-N-乙基铵）-丙基）-2,7-芴）-alt-2,7-（9,9-二辛基芴）]（PFN）溶于水中，形成蓝色发射的聚合物胶束。通过将小的有机荧光团（包括香豆素6（C6）和尼罗红（NR））分别掺入PFN胶束中，可以轻松实现绿色和红色发射。这些胶束在溶液和固体膜中均显示出明亮的荧光，避免了明显的聚集引起的猝灭，并增强了从PFN到C6或NR的荧光共振能量转移。通过一种简单的溶液方法，这些聚合物胶束证明了它们对指纹纹的高度亲和力。因此，获得了具有高分辨率的荧光指纹图像，该荧光指纹图像受到来自基板的荧光背景和相邻指纹之间的重叠的干扰较小。