Trachyspermum ammi and Nigella sativa are mostly used as traditional medicinal plants to cure different diseases. It was observed that both extracts showed significant antibacterial activity against gram-negative bacteria and which may be extended to the SARS-CoV-2, leading to the absence of the peptidoglycan layer. T. ammi showed the highest percentage inhibition in the antifungal assay compared to N. sativa against Aspergillus flavus. The antioxidant assay was performed. It was observed that T. ammi has more free radical scavenging activity as indicated by low IC50. The cytotoxic assay was performed by following brine shrimp lethality assay, and it was observed that T. ammi has more cytotoxicity. The results are that both N. sativa and T. ammi are the origins of active pharmaceutical ingredients and have several pharmacological effects. Furthermore, both essential antibacterial agents were identified and can, therefore, be used as an antibiotic. They have now shown important antioxidant and cytotoxic potential, such that their potential in herbal medicine and cancer genetics can now unravel. In the future, the bioactive molecules responsible for biological processes will be separated. Besides, the nano-formulations in nano-biotechnology may also be analyzed with these extracts.

Trachyspermum ammi和Nigella sativa被广泛用作治疗各种疾病的传统药用植物。观察到两种提取物均显示出对革兰氏阴性细菌的显着抗菌活性，并且可能扩展至SARS-CoV-2，导致不存在肽聚糖层。与苜蓿芽孢杆菌对黄曲霉的抗性比较，T. ammi的抑菌率最高。进行抗氧化剂测定。观察到，如低IC 50所表明的，T。ammi具有更多的自由基清除活性。通过以下盐水虾致死率测定进行细胞毒性测定，观察​​到T.ammi具有更大的细胞毒性。结果是，这两个N.苜蓿和T. AMMI是活性药物成分的来源和具有若干药理作用。此外，已鉴定出两种必需的抗菌剂，因此可以用作抗生素。他们现在显示出重要的抗氧化剂和细胞毒性潜力，因此它们在草药和癌症遗传学方面的潜力现在可以被挖掘出来。将来，负责生物过程的生物活性分子将被分离。此外，也可以用这些提取物分析纳米生物技术中的纳米制剂。