Plant Growth Regulation ( IF 3.5 ) Pub Date : 2021-01-22 , DOI: 10.1007/s10725-020-00685-2 Xudong Zhang , Bastian L. Franzisky , Lars Eigner , Christoph‐Martin Geilfus , Christian Zörb
Chloride (Cl−) is required for photosynthesis and regulates osmotic balance. However, excess Cl− application negatively interacts with nitrate (\({\mathrm{NO}}_{3}^{-}\)) uptake, although its effect on \({\mathrm{NO}}_{3}^{-}\) metabolism remains unclear. The aim was to test whether Cl− stress disturbs nitrate reductase activity (NRA). A maize variety (Zea mays L. cv. LG 30215) was hydroponically cultured in a greenhouse under the following conditions: control (2 mM CaCl2), moderate Cl− (10 mM CaCl2), high Cl− (60 mM CaCl2). To substantiate the effect of Cl− stress further, an osmotic stress with lower intensity was induced by 60 g polyethylene glycol (PEG) 6000 L−1 + 2 mM CaCl2), which was 57% of the osmotic pressure being produced by 60 mM CaCl2. Results show that high Cl− and PEG-induced osmotic stress significantly reduced shoot biomass, stomatal conductance and transpiration rate, but NRA was only decreased by high Cl− stress. The interference of NRA in chloride-stressed maize is supposed to be primarily caused by the antagonistic uptake of Cl− and \({\mathrm{NO}}_{3}^{-}\).
中文翻译:
氯化物和硝酸盐的拮抗作用抑制了氯化物胁迫的玉米中硝酸盐还原酶的活性
氯化物(CL - )所需的光合作用和调节渗透压平衡。然而,过量的氯-应用负硝酸盐相互作用(\({\ mathrm {NO}} _ {3} ^ { - } \) )的摄取,尽管其上作用\({\ mathrm {NO}} _ {3} ^ {-} \)代谢仍不清楚。其目的是测试是否氯-硝酸盐应力会干扰还原酶活性(NRA)。甲玉米品种(玉蜀黍。L. CV LG 30215)在温室中水培在以下条件下:控制(2毫米氯化钙2),中度氯-(10毫米氯化钙2),高氯-(60毫米氯化钙2)。证实Cl组成的效果-进一步应力,具有较低强度的渗透胁迫诱导60克聚乙二醇(PEG)6000大号-1 + 2毫米氯化钙2),这是渗透压的57%是由60mM的产生氯化钙2。结果表明,高氯-和PEG诱导的渗透胁迫显著减少枝条生物量,气孔导度和蒸腾速率,但NRA只减少高氯-应力。NRA的在氯乙烯-强调玉米的干扰应该被主要由Cl组成的拮抗摄取引起-和\({\ mathrm {NO}} _ {3} ^ { - } \) 。