Eco-friendly synthesis of biogenic silver nanoparticles (AgNPs) employing plants is becoming increasingly attractive for biomedical applications including cancer diagnosis and treatment. The present study deals with the biosynthesis of AgNPs using root extract from Annona muricata (AMR), optimization of physico-chemical parameters for the effective synthesis and evaluation of their antioxidant and cytotoxic potential. UV–Vis / FTIR spectroscopy, XRD, FESEM and EDX techniques confirmed the surface plasmon resonance at 440 nm of the crystalline, spherical AgNPs capped with phytoconstituents. AMRAgNPs exhibited strong antioxidant activity also showed selective cytotoxicity against HCT116, without affecting growth of normal human lymphocytes and erythrocytes. Light, fluorescence and scanning electron microscopy revealed apoptosis-related cytomorphological alterations and increase in ROS levels whilst clonogenic assay confirmed reduction in colony formation capacity in AMRAgNPs treated cells. Flow cytometric analysis revealed increase in the sub-G₁cell population indicative of apoptosis induction. The expression profile of the apoptosis-associated genes, PUMA, caspase-3, -8, -9, Bax and Bcl-2 obtained through qRT-PCR, combined with the presence of p53 and p21, cleaved PARP, caspase-3, -9, on western blots unambiguously confirmed occurrence of mitochondrial apoptosis. The present study highlights the selective apoptogenic activity of the A.muricata root extract-derived AgNPs which can serve as a potent anticancer agent for colon cancer.

利用植物的生态友好型生物基因银纳米颗粒（AgNPs）合成对包括癌症诊断和治疗在内的生物医学应用越来越有吸引力。本研究涉及使用栗色Annona muricata的根提取物进行AgNPs的生物合成。（AMR），优化物理化学参数以有效合成和评估其抗氧化剂和细胞毒性潜力。UV-Vis / FTIR光谱，XRD，FESEM和EDX技术证实了被植物成分覆盖的结晶球形AgNP在440 nm处的表面等离子体共振。AMRAgNPs表现出强大的抗氧化活性，还显示出针对HCT116的选择性细胞毒性，而不会影响正常人淋巴细胞和红细胞的生长。光，荧光和扫描电子显微镜显示凋亡相关的细胞形态学改变和ROS水平的增加，而克隆形成测定证实AMRAgNPs处理的细胞中菌落形成能力的降低。流式细胞仪分析显示sub-G _1增加指示凋亡诱导的细胞群。通过qRT-PCR获得的与凋亡相关的基因PUMA，caspase-3，-8，-9，Bax和Bcl-2的表达谱，结合p53和p21的存在，裂解了PARP，caspase-3，-如图9所示，在蛋白质印迹上明确证实了线粒体凋亡的发生。本研究着重指出了毛孔农杆菌根提取物衍生的AgNP的选择性凋亡活性，该AgNP可以作为结肠癌的有效抗癌剂。