The structures of arthropod bacterial communities are complex. These microbiotas usually provide many beneficial services to their hosts, whereas occasionally they may be parasitical. To date, little is known about the bacterial communities of Tetranychus truncatus and the factors contributing to the structure of its bacterial communities are unexplored yet. Here, we used four symbiont-infected T. truncatus strains—including one Wolbachia and Spiroplasma co-infected strain, two symbiont singly-infected strains and one symbiont uninfected strain—to investigate the influence of endosymbionts on the structure of the host mites’ microbiota. Based on 16S rRNA genes sequencing analysis, we found Wolbachia and Spiroplasma were the two most abundant bacteria in T. truncatus and the presence of both symbionts could not change the diversity of bacterial communities (based on alpha-diversity indexes such as ACE, Chao1, Shannon and Simpson diversity index). Symbiont infection did alter the abundance of many other bacterial genera, such as Megamonas and Bacteroides. The structures of bacterial communities differed significantly among symbiont-infected strains. These results suggested a prominent effect of Wolbachia and Spiroplasma on bacterial communities of the host T. truncatus. These findings advance our understanding of T. truncatus microbiota and will be helpful for further study on bacterial communities of spider mites.

节肢动物细菌群落的结构很复杂。这些微生物群通常会为其宿主提供许多有益的服务，而有时它们可​​能是寄生的。迄今为止，人们对四叶螨（Tetranychus truncatus）的细菌群落知之甚少，并且尚未对其构成其细菌群落结构的因素进行探索。在这里，我们使用了四个共生体感染的牛逼。truncatus菌株（包括一种Wolbachia和Spiroplasma共同感染的菌株，两种共生菌单独感染的菌株和一种共生菌未感染的菌株）研究内共生菌对宿主螨微生物群结构的影响。基于16S rRNA基因测序分析，我们发现Wolbachia和Spiroplasma是T中两个最丰富的细菌。截肢和两个共生体的存在都不能改变细菌群落的多样性（基于ACE，Chao1，Shannon和Simpson多样性指数等alpha多样性指数）。共生菌感染确实改变了许多其他细菌属的丰度，例如大摩纳哥和拟杆菌。在共生菌感染菌株之间，细菌群落的结构差异显着。这些结果表明，Wolbachia和Spiroplasma对寄主T的细菌群落具有显著作用。run。这些发现促进了我们对T的理解。run微生物群，将有助于进一步研究红蜘蛛的细菌群落。