As recently announced by the American Society for Reproductive Medicine (ASRM), human ovarian tissue cryopreservation is an established option for fertility preservation in prepubertal girls and young women undergoing gonadotoxic treatments for cancer as well as some autoimmune diseases. Proper ovarian tissue assessment before and after cryopreservation is essential to increase success rates. Ovarian fragments from 16 patients were divided into small pieces in form of cortex with medulla, and randomly divided into the following two groups. Pieces of Group 1 (n = 16) were frozen immediately after operation, thawed and just after thawing their quality was analyzed. Group 2 pieces (n = 16) after operation were cooled to 5 °C for 24 h, then frozen after 24 h pre-cooling to 5 °C, thawed and just after thawing their quality was analyzed. The effectiveness of the pre-freezing cooling of tissue was evaluated by the development and viability of follicles (Calcein-AM and Propidium Iodide) using complex object parametric analyzer and sorter machine (COPAS). Positive effect of cooling of cells to low supra-zero temperatures on their future development after re-warming has been observed. New flow cytometry- technique is suitable for the evaluation and sorting of cryopreserved whole human whole intact ovarian fragments. Long time (24 h) cooling of ovarian tissue to 5 °C before cryopreservation has a trend of a cell viability increasing.

正如美国生殖医学协会 (ASRM) 最近宣布的那样，人类卵巢组织冷冻保存是青春期前女孩和年轻女性接受癌症和某些自身免疫性疾病的性腺毒性治疗的生育能力保存的既定选择。在冷冻保存之前和之后进行适当的卵巢组织评估对于提高成功率至关重要。将16例患者的卵巢碎片分成带髓质皮质的小块，随机分为以下两组。第 1 组 ( n  = 16) 的切片在手术后立即冷冻、解冻并在解冻后对其质量进行分析。组 2 件 ( n = 16) 运行后冷却至5°C 24 h，然后冷冻24 h 后预冷至5°C，解冻并在解冻后对其质量进行分析。使用复杂对象参数分析仪和分选机 (COPAS) 通过卵泡（Calcein-AM 和碘化丙啶）的发育和存活率来评估组织预冷冻冷却的有效性。已经观察到将细胞冷却至低于零的温度对其重新加热后的未来发展产生积极影响。新的流式细胞仪技术适用于冷冻保存的整个人类完整的卵巢碎片的评估和分选。在冷冻保存前长时间（24小时）将卵巢组织冷却至5°C具有细胞活力增加的趋势。