This work sought to understand relation between the variations in chromatic values of biomass during different pretreatments and substrate digestibility. The giant reed (Arundo donax) was subjected to hot water (HW) and dilute acid (DA) aqueous (0.5%, w/w, H₂SO₄) with elevated temperature. The effects of the pretreatments on the chemical, structure, enzymatic hydrolysis efficiency, and color traits of the giant reed were investigated. Results from the colorimetric system (CIE-L^*a^*b^*) showed that the luminance (∆L^*) decreased with temperature for all conditions, and the total color difference (∆E) was dramatically changed during DA pretreatment. The ∆E showed a correlation with the content of the lignin (R² = 0.76) and xylan (R² = 0.80). BET (Brunauer-Emmett-Teller) analysis and the images obtained from microscopes confirmed that the porosity of the cell wall increased with the severity of the pretreatments. The 180-DA-pretreated samples exhibited satisfactory enzymatic hydrolysis efficiency with 99.0%. There was a positive correlation between ∆E and enzymatic hydrolysis efficiency, which could serve as rough yet high-throughput judges for predicting the trend of saccharification efficiency of the giant reed.

这项工作试图了解不同预处理期间生物量色度值变化与底物消化率之间的关系。使巨型芦苇（Arundo donax）在高温下经受热水（HW）和稀酸（DA）水溶液（0.5％，w / w，H ₂ SO ₄）的作用。研究了预处理对巨型芦苇的化学，结构，酶水解效率和颜色特征的影响。比色系统（CIE-L ^* a ^* b ^*）的结果表明，亮度（∆L ^*）在所有条件下均随温度降低，并且在DA预处理期间总色差（ΔE）发生了显着变化。∆E与木质素（R ² = 0.76）和木聚糖（R ² = 0.80）的含量相关。BET（Brunauer-Emmett-Teller）分析和从显微镜获得的图像证实，细胞壁的孔隙度随预处理的严重性而增加。经180-DA预处理的样品显示出令人满意的酶解效率，为99.0％。ΔE与酶解效率之间存在正相关，可以作为粗略而高通量的判断标准，以预测巨型芦苇的糖化效率趋势。