Isoishwarane synthase from Streptomyces lincolnensis,Organic Chemistry Frontiers

当前位置： X-MOL 学术 › Org. Chem. Front. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Isoishwarane synthase from Streptomyces lincolnensis
Organic Chemistry Frontiers ( IF 4.6 ) Pub Date : 2021-1-19 , DOI: 10.1039/d0qo01583k
Houchao Xu _{1,

2,

3,

4} , Jan Rinkel _{1,

2,

3,

4} , Jeroen S. Dickschat _{1,

2,

3,

4}

Affiliation

A new sesquiterpene synthase from Streptomyces lincolnensis was identified as isoishwarane synthase. The absolute configuration of its product was determined by an enantioselective deuteration strategy. The cyclisation mechanism from farnesyl diphosphate to isoishwarane was studied in incubation experiments with various isotopically labelled substrates and by site-directed mutagenesis. GC/MS analysis of all 15 isotopomers of (¹³C)isoishwarane also gave insights into the EI-MS fragmentation mechanism.

中文翻译：

林肯链霉菌的异ishwarwarane合酶

来自林肯链霉菌的一种新的倍半萜烯合酶被鉴定为异异戊烷合酶。其产品的绝对构型由对映选择性氘化策略确定。在与各种同位素标记底物的孵育实验中，并通过定点诱变，研究了从法呢基二磷酸酯到异二十烷的环化机理。（¹³ C）异ishwarwarane的所有15个异构体的GC / MS分析也为EI-MS片段化机理提供了见解。

更新日期：2021-01-22

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11