The present study was designed to investigate the therapeutic effects against oxidative stress and alleviative effects of Peganum harmala seeds (PH) in rats with Complete Freund’s Adjuvant (CFA) induced arthritis. The extract was evaluated for its phytoconstituents, antiarthritic and antioxidant properties. The action of chloroform (PHC) and ethanolic (PHE) extracts of PH was evaluated in adult Lewis rats (150-200 g).with CFA induced arthritis. Arthritic rats received PH extracts 100 mg/kg orally for 28 consecutive days (Prophylactic treatment) and from 14th day of CFA injection (Therapeutic treatment). PHE significantly suppressed the arthritis severity in rats than PHC in 28 days. All complications shown significant reduction (p< 0.05) in arthritic rats including paw volume (63.09%), body weight loss, decreased locomotor activity, erythrocyte sedimentation rate and synovial/hepatic tissues lipid peroxidation and increase in cellular antioxidants superoxide dismutase (U/mg) activities and hemoglobin counts. The results showed the presence of alkaloids and flavonoids in PHE. Histology and radiographic analysis of arthritic ankle joints indicated abnormal changes. Marked reduction in inflammation and arthritic changes were observed after treatment with PHE. Therefore, the investigation suggests that PHE at 100 mg/kg will be useful in the management of rheumatoid arthritis complications which may possibly be due to boosting the intracellular antioxidant defense.

中文翻译：

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骆驼蓬种子改善实验性关节炎的氧化和炎症变化。

本研究旨在研究完全弗氏佐剂（CFA）诱发的关节炎大鼠抗氧化应激的作用以及对决明子种子（PH）的缓解作用。评价该提取物的植物成分，抗关节炎和抗氧化特性。在成年Lewis大鼠（150-200 g）中，用CFA诱发的关节炎评估了PH的氯仿（PHC）和乙醇（PHE）提取物的作用。关节炎大鼠连续28天（预防性治疗）和CFA注射第14天（治疗性治疗）口服100 mg / kg的PH提取物。与PHC相比，PHE在28天内显着抑制了大鼠的关节炎严重程度。所有并发症均显示关节炎大鼠明显减少（p <0.05），包括爪子体积（63.09％），体重减轻，运动能力降低，红细胞沉降率和滑膜/肝组织脂质过氧化以及细胞抗氧化剂超氧化物歧化酶（U / mg）活性和血红蛋白计数增加。结果表明，PHE中存在生物碱和类黄酮。关节炎踝关节的组织学和影像学分析表明异常变化。用PHE治疗后观察到炎症和关节炎改变明显减少。因此，研究表明100 mg / kg的PHE在类风湿关节炎并发症的治疗中可能是有用的，这可能是由于增强了细胞内抗氧化剂的防御能力所致。结果表明，PHE中存在生物碱和类黄酮。关节炎踝关节的组织学和影像学分析表明异常变化。用PHE治疗后观察到炎症和关节炎改变明显减少。因此，研究表明100 mg / kg的PHE在类风湿关节炎并发症的治疗中可能是有用的，这可能是由于增强了细胞内抗氧化剂的防御能力所致。结果表明，PHE中存在生物碱和类黄酮。关节炎踝关节的组织学和影像学分析表明异常变化。用PHE治疗后观察到炎症和关节炎改变明显减少。因此，研究表明100 mg / kg的PHE在类风湿关节炎并发症的治疗中可能是有用的，这可能是由于增强了细胞内抗氧化剂的防御能力所致。