Campylobacter spp. enteritis is the most frequent bacterial enteritis in both adults and children and is sometimes a source of severe complications. Its diagnosis by culture suffers from a lack of sensitivity and delays the result, preventing an early initiation of optimal antibiotic therapy in some cases. Our aim was to test a new rapid immuno-enzymatic method for Campylobacter spp. diagnosis in comparison to a composite reference standard (CRS). Stool samples from the French National Reference Center for Campylobacter and Helicobacter were tested with the CAMPYLOBACTER QUIK CHEK™ (Abbott). The CRS used to consider a case positive for Campylobacter spp. was a positive culture and, in case of a negative culture, a positive result obtained with both an ELISA and a molecular test. One hundred and eight stools were included: 53 were positive according to the CRS. If performed alone, culture would have missed 5 cases which the CAMPYLOBACTER QUIK CHEK™ detected. Finally, the CAMPYLOBACTER QUIK CHEK™ showed a sensitivity of 96.2% and a specificity of 94.5% and is relevant for clinical practice. Given the characteristics of the new method, it can be used as a screening method for Campylobacter spp. detection.

中文翻译：

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评估CAMPYLOBACTER QUIK CHEK™快速膜酶免疫测定法，以检测弯曲杆菌属。粪便样本中的抗原

弯曲杆菌属。肠炎是成人和儿童中最常见的细菌性肠炎，有时是严重并发症的来源。其通过培养物的诊断缺乏敏感性并延误了结果，在某些情况下阻止了早期开始最佳抗生素治疗。我们的目的是测试弯曲杆菌属的一种新的快速免疫酶法。与综合参考标准（CRS）相比诊断。来自法国国家弯曲杆菌和幽门螺杆菌参考中心的粪便样品用CAMPYLOBACTER QUIK CHEK™（Abbott）测试。CRS过去曾认为弯曲杆菌属细菌呈阳性。为阳性培养，如果为阴性培养，则通过ELISA和分子检测获得阳性结果。包括一百零八份粪便：根据CRS，有53例阳性。如果单独进行培养，将错过5例CAMPYLOBACTER QUIK CHEK™检测到的病例。最后，CAMPYLOBACTER QUIK CHEK™显示出96.2％的敏感性和94.5％的特异性，与临床实践有关。鉴于新方法的特点，它可用作弯曲杆菌属的筛选方法。检测。