A helical amplification system composed of artificial nucleic acids,Chemical Science

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A helical amplification system composed of artificial nucleic acids
Chemical Science ( IF 7.6 ) Pub Date : 2021-1-12 , DOI: 10.1039/d0sc05245k
Hiromu Kashida ₁ , Keiji Nishikawa ₁ , Wenjing Shi ₁ , Toshiki Miyagawa ₁ , Hayato Yamashita ₂ , Masayuki Abe ₂ , Hiroyuki Asanuma ₁

Affiliation

Herein we report an amplification system of helical excess triggered by nucleic acid hybridization for the first time. It is usually impossible to prepare achiral nanostructures composed of nucleic acids because of their intrinsic chirality. We used serinol nucleic acid (SNA) oligomers for the preparation of achiral nanowires because SNA oligomers with symmetrical sequences are achiral. Nanowire formation was confirmed by atomic force microscopy and size exclusion chromatography. When a chiral nucleic acid with a sequence complementary to SNA was added to the nanostructure, helicity was induced and a strong circular dichroism signal was observed. The SNA nanowire could amplify the helicity of chiral nucleic acids through nucleobase stacks. The SNA nanostructures have potential for use as platforms to detect chiral biomolecules under aqueous conditions because SNA can be readily functionalized and is water-soluble.

中文翻译：

由人工核酸组成的螺旋扩增系统

在此，我们首次报道了由核酸杂交触发的螺旋过量的扩增系统。由于其固有的手性，通常不可能制备由核酸组成的非手性纳米结构。我们使用丝氨醇核酸（SNA）寡聚体来制备非手性纳米线，因为具有对称序列的SNA寡聚体是非手性的。通过原子力显微镜和尺寸排阻色谱法确认了纳米线的形成。当将具有与SNA互补的序列的手性核酸添加至纳米结构时，诱导了螺旋性并且观察到了强的圆二色性信号。SNA纳米线可以通过核碱基堆栈放大手性核酸的螺旋度。

更新日期：2021-01-22

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