To compare neuroimaging data between subjects, images from individual sessions need to be aligned to a common reference or “atlas.” Atlas registration of optical intrinsic signal imaging of mice, for example, is commonly performed using affine transforms with parameters determined by manual selection of canonical skull landmarks. Errors introduced by such procedures have not previously been investigated. We quantify the variability that arises from this process and consequent errors from misalignment that affect interpretation of functional neuroimaging data. We propose an improved method, using separately acquired high-resolution images and demonstrate improvements in variability and alignment using this method.

中文翻译：

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光学固有信号成像图谱配准的变异性及其对功能连接分析的影响


为了比较受试者之间的神经影像数据，来自各个会话的图像需要与通用参考或“图集”对齐。例如，小鼠光学内在信号成像的图谱配准通常使用仿射变换来执行，其参数是通过手动选择规范头骨标志来确定的。以前没有调查过此类程序引入的错误。我们量化了这一过程中产生的变异性以及因未对准而产生的影响功能神经影像数据解释的随之而来的错误。我们提出了一种改进的方法，使用单独采集的高分辨率图像，并展示了使用该方法在可变性和对齐方面的改进。