Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode,Bioconjugate Chemistry

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Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2021-01-22 , DOI: 10.1021/acs.bioconjchem.0c00657
Akira Matsumoto _{1,

2} , Shigehito Osawa ₃ , Takahiro Arai ₁ , Yukie Maejima ₄ , Hidenori Otsuka _{3,

4,

5,

6} , Yuji Miyahara ₁

Affiliation

Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation. Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states. A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was “grafted-to” a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide–alkyne cycloaddition reaction. Using the electrode, fetuin was potentiometrically detectable with a μM-order-sensitivity that is comparable to what is found in blood-collected specimen. Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.

中文翻译：

硼酸末端功能化聚（乙二醇）修饰电极对循环糖蛋白的电位测定

尽管聚糖结构极其复杂，但唾液酸 (SA) 由于其独特的阴离子性质和聚糖链末端占据，为糖基化提供了一个可分析的指标。利用基于硼酸 (BA) 的 SA 识别化学，我们在这里展示了胎球蛋白的无标记、无酶、电位测定，胎球蛋白是一种与生理和各种病理状态有关的血液循环糖蛋白。苯基硼酸 (PBA) ω-末端官能化聚乙二醇 (PEG) 与带有悬垂炔基的 α-束缚单元“接枝”到用 11-叠氮化物-十一硫醇修饰的金电极上，通过铜催化叠氮化物-炔环加成反应。使用电极，胎球蛋白是可电位检测的，其灵敏度为 μM 级，与血液采集标本中发现的灵敏度相当。我们的发现可能对开发一种易于缩小规模和大规模生产的显着经济的血液诊断技术具有重要意义。

更新日期：2021-02-17

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