Colonization by the benign tapeworm, Hymenolepis diminuta, has been associated with a reduction in intestinal inflammation and changes in bacterial microbiota. However, the role of microbiota in the tapeworm anti-inflammatory effect is not yet clear, and the aim of this study was to determine whether disruption of the microflora during worm colonization can affect the course of intestinal inflammation. We added a phase for disrupting the intestinal microbiota using antibiotics to the experimental design for which we previously demonstrated the protective effect of H. diminuta. We monitored the immunological markers, clinical parameters, bacterial microbiota, and histological changes in the colon of rats. After a combination of colonization, antibiotics, and colitis induction, we had four differently affected experimental groups. We observed a different course of the immune response in each group, but no protective effect was found. Rats treated with colonization and antibiotics showed a strong induction of the Th2 response as well as a significant change in microbial diversity. The microbial results also revealed differences in the richness and abundance of some bacterial taxa, influenced by various factors. Our data suggest that interactions between the tapeworm and bacteria may have a major impact on its protective effect.

中文翻译：

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蠕虫与宿主肠道细菌的相互作用对于其免疫调节作用至关重要

良性tape虫Hymenolepis diminuta的定殖与肠道炎症的减少和细菌微生物群的改变有关。然而，微生物群在the虫抗炎作用中的作用尚不明确，本研究的目的是确定蠕虫定殖过程中微生物区系的破坏是否会影响肠道炎症过程。我们在实验设计中增加了一个使用抗生素破坏肠道菌群的阶段，之前我们已经证明了它的保护作用。。我们监测了大鼠结肠中的免疫标志物，临床参数，细菌菌群和组织学变化。在定植，抗生素和诱导结肠炎相结合后，我们有四个受影响的实验组。我们在每组中观察到不同的免疫反应过程，但是未发现保护作用。用定植和抗生素治疗的大鼠表现出对Th2反应的强烈诱导以及微生物多样性的显着变化。微生物结果还揭示了受各种因素影响的某些细菌类群的丰富度和丰度差异。我们的数据表明，the虫和细菌之间的相互作用可能对其保护作用产生重大影响。