Abstract

Purpose

In a nuclear or radiological event, an early diagnostic tool is needed to distinguish the worried well from those individuals who may later develop life-threatenFing hematologic acute radiation syndrome. We examined the contribution of the peripheral blood's cell populations on radiation-induced gene expression (GE) changes.

Materials and methods

EDTA-whole-blood from six healthy donors was X-irradiated with 0 and 4Gy and T-lymphocytes, B-lymphocytes, NK-cells and granulocytes were separated using immunomagnetic methods. GE were examined in cell populations and whole blood.

Results

The cell populations contributed to the total RNA amount with a ratio of 11.6 for T-lymphocytes, 1.2 for B-cells, 1.2 for NK-cells, 1.0 for granulocytes. To estimate the contribution of GE per cell population, the baseline (0Gy) and the radiation-induced fold-change in GE relative to unexposed was considered for each gene. The T-lymphocytes (74.8%/80.5%) contributed predominantly to the radiation-induced up-regulation observed for FDXR/DDB2 and the B-lymphocytes (97.1%/83.8%) for down-regulated POU2AF1/WNT3 with a similar effect on whole blood gene expression measurements reflecting a corresponding order of magnitude.

Conclusions

T- and B-lymphocytes contributed predominantly to the radiation-induced up-regulation of FDXR/DDB2 and down-regulation of POU2AF1/WNT3. This study underlines the use of FDXR/DDB2 for biodosimetry purposes and POU2AF1/WNT3 for effect prediction of acute health effects.

中文翻译：

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受辐射外周血细胞群中急性辐射综合征相关基因的表达

 抽象的

 目的

在核或放射事件中，需要一种早期诊断工具来区分那些担心的人和后来可能出现危及生命的血液学急性辐射综合症的人。我们检查了外周血细胞群对辐射诱导的基因表达（GE）变化的影响。

 材料和方法

来自六名健康供体的 EDTA 全血用 0 和 4Gy 进行 X 射线照射，并使用免疫磁性方法分离 T 淋巴细胞、B 淋巴细胞、NK 细胞和粒细胞。对细胞群和全血中的 GE 进行了检查。

 结果

细胞群对总 RNA 量的贡献比例为：T 淋巴细胞为 11.6，B 细胞为 1.2，NK 细胞为 1.2，粒细胞为 1.0。为了估计每个细胞群的 GE 的贡献，考虑了每个基因的基线 (0Gy) 和辐射引起的 GE 相对于未暴露的倍数变化。 T 淋巴细胞 (74.8%/80.5%) 主要导致辐射诱导的FDXR/DDB2上调，B 淋巴细胞 (97.1%/83.8%) 导致POU2AF1/WNT3 下调，对辐射诱导的 POU2AF1/WNT3也有类似影响。全血基因表达测量反映了相应的数量级。

 结论

T淋巴细胞和B淋巴细胞主要导致辐射诱导的FDXR/DDB2上调和POU2AF1/WNT3下调。本研究强调使用FDXR/DDB2进行生物剂量测定，使用POU2AF1/WNT3进行急性健康影响的效果预测。