Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method,Phytochemical Analysis

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Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC-QTRAP-MS/MS method
Phytochemical Analysis ( IF 3.0 ) Pub Date : 2021-01-21 , DOI: 10.1002/pca.3024
Jun Xu _{1,

2} , Rui Wang _{1,

2} , Junlin Liu _{1,

2} , He Cheng _{1,

2} , Daiyin Peng _{1,

2} , Lihua Xing _{1,

2} , Suying Shi _{1,

2} , Nianjun Yu _{1,

2}

Affiliation

Pre-column derivatisation using 1-phenyl-3-methyl-5-pyrazolone (PMP) is a common method for monosaccharide determination. Herein, a specific ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP).

中文翻译：

高效 UHPLC-QTRAP-MS/MS 方法测定枸杞果实多糖中的单糖

使用 1-苯基-3-甲基-5-吡唑啉酮 (PMP) 进行柱前衍生是单糖测定的常用方法。在此，开发了一种特定的超高效液相色谱四极杆捕集串联质谱 (UHPLC-QTRAP-MS/MS) 方法，用于测定枸杞多糖 (LBP)的单糖组成。

更新日期：2021-01-21

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