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Bestrophin-derived peptide, WP17, elicits cell wall disruption-mediated bactericidal activity against Micrococcus luteus and anti-neoplastic effect against murine melanoma cells
Peptide Science ( IF 1.5 ) Pub Date : 2021-01-22 , DOI: 10.1002/pep2.24220
Gayathri Ravichandran 1 , Stefi V. Raju 1 , Purabi Sarkar 1 , Saraswathi N. T. 2 , Ebtesam M. Al Olayan 3 , Abeer S. Aloufi 3 , Mohamed A. Elokaby 4 , Aziz Arshad 5, 6 , Kanchana Mala 7 , Jesu Arockiaraj 1
Affiliation  

The cDNa sequence of Bestrophin-1 (BEST-1) was identified from a previously constructed transcriptome data set of freshwater prawn Macrobrachium rosenbergii (Mr). Basal and temporal gene expression analysis of MrBEST-1 showed its antimicrobial immune effectiveness during viral and bacterial infections. The protein sequence encoded by cDNA of MrBEST-1 was examined and a short antimicrobial molecule, named WP17 was identified using a bioinformatics tool. Further, the antibacterial ability of the identified WP17 peptide was evaluated against a number of bacterial strains, in which the peptide showed potential bactericidal activity against Micrococcus luteus (MTCC 6164), Staphylococcus aureus (ATCC 9144), Escherichia coli (ATCC 9637), Klebsiella pneumonia (CI 7376) and Bacillus subtilis (ATCC 6051). Based on the results, further assays focused on M. luteus MTCC 6164. The mode of action of MrWP17 on M. luteus MTCC 6164 was analyzed using FACS and FESEM. Toxicity analysis suggested that WP17 impaired the viability of cells in murine melanoma cells (B16F10); however, no cytotoxicity was observed against kidney embryonic cells (HEK293), even at higher concentrations. Similarly, the gene expression analysis of WP17 peptide treated murine cells elicited an extrinsic apoptotic pathway. In the present study, we have demonstrated the involvement of MrBEST-1 in immune mechanisms through its short peptide molecule that has potential antimicrobial activity.

中文翻译:

Bestrophin 衍生肽 WP17 引发细胞壁破坏介导的对藤黄微球菌的杀菌活性和对鼠黑色素瘤细胞的抗肿瘤作用

Bestrophin-1 (BEST-1) 的 cDNA 序列是从先前构建的淡水虾罗氏沼虾( Mr )转录组数据集中鉴定的。BEST-1先生的基础和时间基因表达分析显示其在病毒和细菌感染期间的抗菌免疫效力。对BEST-1先生的 cDNA 编码的蛋白质序列进行了检查,并使用生物信息学工具鉴定了一个名为 WP17 的短抗菌分子。此外,鉴定的 WP17 肽对多种细菌菌株的抗菌能力进行了评估,其中该肽对藤黄微球菌(MTCC 6164)、金黄色葡萄球菌显示出潜在的杀菌活性(ATCC 9144)、大肠杆菌(ATCC 9637)、肺炎克雷伯菌(CI 7376) 和枯草芽孢杆菌(ATCC 6051)。基于这些结果,进一步的测定集中在藤黄微球菌MTCC 6164.的作用方式先生WP17上藤黄微球菌使用FACS和FESEM分析MTCC 6164。毒性分析表明,WP17 削弱了鼠黑色素瘤细胞 (B16F10) 中细胞的活力;然而,即使在较高浓度下,也未观察到对肾胚胎细胞 (HEK293) 的细胞毒性。类似地,WP17 肽处理的小鼠细胞的基因表达分析引发了外源性凋亡途径。在本研究中,我们已经证明了先生的参与BEST-1 通过其具有潜在抗菌活性的短肽分子在免疫机制中发挥作用。
更新日期:2021-01-22
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