Landfills are becoming the most common way to dispose textiles. The presence of different types of dyes and finishes on textiles fabrics can become an important source of pollution during the degradation process, due to the fact that these chemicals can leach to the soil. The biodegradation of dyes from dyed fabrics is not fully understood, and what can leach into the soil can be more toxic. In this study, cotton fabrics were dyed with CI Reactive Blue 19 (RB19) and biodegraded in soil in a laboratory‐controlled environment for a 90‐day time interval by using the ASTM D 5988‐18 method. A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction method, in combination with liquid chromatography‐mass spectrometry (LC‐MS) analysis, was applied to soil containing RB19 after the degradation process. An unknown degradation product, with a mass‐to‐charge ratio (m/z) of 393, found in the soil was characterised and confirmed, via high‐resolution MS and tandem MS, to be CI Acid Blue 25 (AB25). To quantify the confirmed unknown product extracted from the degraded soil sample, a quantitation method was developed using high‐performance liquid chromatography‐diode array detector‐mass spectrometry (HPLC‐DAD‐MS). The quantitation method provided excellent linearity (R² = 0.9990 ± 0.0006), accuracy (mean percentage error = 5.17 ± 1.88), precision (mean percentage coefficient of variation = 4.73 ± 4.16), and sensitivity (lower limit of quantitation = 1.29 ± 0.47 µg/mL) for nine concentrations ranging from 0.5 to 40 µg/mL.

中文翻译：

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土壤中CI活性蓝19染料降解产物的鉴定和定量

垃圾填埋场正成为处理纺织品的最常见方式。由于这些化学物质会渗入土壤，因此在纺织品降解过程中，不同类型的染料和整理剂的存在会成为重要的污染源。人们还不完全理解染料从染色织物中的生物降解作用，而渗入土壤的物质可能更具毒性。在这项研究中，棉织物用CI活性蓝19（RB19）染色，并在实验室控制的环境中使用ASTM D 5988-18方法在土壤中生物降解90天。改良的QuEChERS（快速，简便，便宜，有效，坚固和安全）提取方法与液相色谱-质谱（LC-MS）分析相结合，在降解过程后应用于含RB19的土壤。未知的降解产物，在土壤中发现的m / z）为393，并通过高分辨率MS和串联MS鉴定并确认为CI Acid Blue 25（AB25）。为了量化从降解的土壤样品中提取的确定的未知产物，使用高效液相色谱-二极管阵列检测器-质谱（HPLC-DAD-MS）开发了一种定量方法。定量方法具有出色的线性（R ²  = 0.9990±0.0006），准确性（平均百分比误差= 5.17±1.88），精度（平均变异系数= 4.73±4.16）和灵敏度（定量下限= 1.29±0.47） µg / mL）的九种浓度，范围从0.5到40 µg / mL。