Boron (B) is an essential mineral element for plant growth, and the seed B pool of crops can be crucial when seedlings need to establish on low-B soils. To date, it is poorly understood how B accumulation in grain crops is genetically controlled. Here, we assessed the genotypic variation of the B concentration in grains of a spring barley (Hordeum vulgare L.) association panel that represents broad genetic diversity. We found a large genetic variation of the grain B concentration and detected in total 23 quantitative trait loci (QTLs) using genome-wide association mapping. HvNIP2;2/HvLsi6, encoding a potential B-transporting membrane protein, mapped closely to a major-effect QTL accounting for the largest proportion of grain B variation. Based on transport studies using heterologous expression systems and gene expression analysis, we demonstrate that HvNIP2;2/HvLsi6 represents a functional B channel and that expression variation in its transcript level associates with root and shoot B concentrations as well as with root dry mass formation under B-deficient conditions. This article is protected by copyright. All rights reserved.

中文翻译：

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全基因组关联映射确定了 HvNIP2 ;2 / HvLsi6 解释了大麦中有效的硼运输

硼 (B) 是植物生长必不可少的矿物质元素，当幼苗需要在低 B 土壤上生长时，作物的种子 B 库可能至关重要。迄今为止，人们对粮食作物中 B 的积累是如何受到基因控制的了解甚少。在这里，我们评估了代表广泛遗传多样性的春大麦（Hordeum vulgare L.）关联面板中 B 浓度的基因型变异。我们发现谷物 B 浓度存在很大的遗传变异，并使用全基因组关联作图在总共 23 个数量性状基因座 (QTL) 中检测到。HvNIP2;2/HvLsi6 编码一种潜在的 B 转运膜蛋白，与占谷物 B 变异比例最大的主要效应 QTL 密切相关。基于使用异源表达系统和基因表达分析的转运研究，我们证明 HvNIP2;2/HvLsi6 代表功能性 B 通道，其转录水平的表达变化与根和芽 B 浓度以及 B 缺乏条件下根干物质形成相关。本文受版权保护。版权所有。