Silencing of a LIM gene in cotton exhibits enhanced resistance against Apolygus lucorum,Journal of Cellular Physiology

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Silencing of a LIM gene in cotton exhibits enhanced resistance against Apolygus lucorum
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2021-01-22 , DOI: 10.1002/jcp.30281
Sijia Liang _{1,

2,

3} , Jing Luo ₂ , Muna Alariqi ₁ , Zhongping Xu ₁ , Aoli Wang ₂ , Muhammad Naeem Zafar ₁ , Jun Ren ₄ , Fuqiu Wang ₁ , Xuefei Liu ₁ , Yanfeng Xin ₁ , Haonan Xu ₄ , Weifeng Guo ₅ , Yanqin Wang ₅ , Weihua Ma ₁ , Lizhen Chen ₄ , Keith Lindsey ₆ , Xianlong Zhang ₁ , Shuangxia Jin ₁

Affiliation

Plant bugs (Miridae species) have become major agricultural pests that cause increasing and severe economic damage. Plant‐mediated RNA interference (RNAi) is emerging as an eco‐friendly, efficient, and reliable strategy for pest management. In this study, we isolated and characterized a lethal gene of Apolygus lucorum and named it Apolygus lucorum LIM (AlLIM), which produced A. lucorum mortality rates ranging from 38% to 81%. Downregulation of the AlLIM gene expression in A. lucorum by injection of a double‐stranded RNA (dsRNA) led to muscle structural disorganization that resulted in metamorphosis deficiency and increased mortality. Then we constructed a plant expression vector that enabled transgenic cotton to highly and stably express dsRNA of AlLIM (dsAlLIM) by Agrobacterium‐mediated genetic transformation. In the field bioassay, dsAlLIM transgenic cotton was protected from A. lucorum damage with high efficiency, with almost no detectable yield loss. Therefore, our study successfully provides a promising genetically modified strategy to overpower A. lucorum attack.

中文翻译：

棉花中 LIM 基因的沉默表现出对 Apolygus lucorum 的增强抗性

植物臭虫（Miridae 物种）已成为主要的农业害虫，造成越来越严重的经济损失。植物介导的 RNA 干扰 (RNAi) 正在成为一种环保、高效和可靠的害虫管理策略。在这项研究中，我们分离和表征的致死基因Apolygus蝽并把它命名Apolygus蝽LIM（AlLIM），其产生的绿盲蝽死亡率范围从38％至81％。A. lucorum 中AlLIM基因表达的下调通过注射双链 RNA (dsRNA) 导致肌肉结构紊乱，导致变态缺陷和死亡率增加。然后我们构建了一个植物表达载体，通过农杆菌介导的遗传转化，使转基因棉花能够高稳定表达AlLIM的dsRNA （dsAlLIM）。在田间生物测定中，dsAlLIM转基因棉花高效地免受A. lucorum损害，几乎没有可检测到的产量损失。因此，我们的研究成功地提供了一种有前途的转基因策略来压制 A. lucorum攻击。

更新日期：2021-01-22

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